Two independent type III secretion mechanisms for YopE in Yersinia enterocolitica

Pathogenic Yersinia species escape the infected host’s defense mechanisms by targeting cytotoxic Yop proteins into the cytoplasm of macrophages via a type III secretion pathway. Two separate secretion signals contained in YopE were identified, each of which were sufficient but not necessary for the...

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Bibliographic Details
Published in:Molecular microbiology 1997-05, Vol.24 (4), p.757-765
Main Authors: Cheng, Luisa W., Anderson, Deborah M., Schneewind, Olaf
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Outer Membrane Proteins - metabolism
Bacterial Proteins - metabolism
Bacterial Proteins - physiology
Biological Transport
Sequence Deletion
Structure-Activity Relationship
Trans-Activators - metabolism
Trans-Activators - physiology
Yersinia enterocolitica
Yersinia enterocolitica - pathogenicity
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Summary:Pathogenic Yersinia species escape the infected host’s defense mechanisms by targeting cytotoxic Yop proteins into the cytoplasm of macrophages via a type III secretion pathway. Two separate secretion signals contained in YopE were identified, each of which were sufficient but not necessary for the secretion of reporter molecules. One signal is located within the coding sequence of the first 15 amino acids and is sufficient for the secretion of fusion proteins but not required for YopE secretion. The second signal is located downstream at residues 15–100 of YopE and is only recognized by the type III machinery when it is bound to SycE. We propose the existence of two independent mechanisms that allow for the secretion of Yop proteins.
ISSN:0950-382X
1365-2958
DOI:10.1046/j.1365-2958.1997.3831750.x