Inhibition of Ran Guanosine Triphosphatase-Dependent Nuclear Transport by the Matrix Protein of Vesicular Stomatitis Virus

Transport of macromolecules into and out of nuclei, essential steps in gene expression, are potential points of control. The matrix protein (M protein) of vesicular stomatitis virus (VSV) was shown to block transport of RNAs and proteins between the nucleus and cytoplasm of Xenopus laevis oocytes. T...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1997-06, Vol.276 (5320), p.1845-1848
Main Authors: Her, Lu-Shiun, Lund, Elsebet, Dahlberg, James E.
Format: Article
Language:English
Subjects:
alpha Karyopherins
Animals
Antibodies
Biological and medical sciences
Biological transport
Biological Transport, Active
Cell Nucleus - metabolism
Cell regulation
Cellular control mechanisms
Coding
Cytoplasm
Cytoplasm - metabolism
Fundamental and applied biological sciences. Psychology
Gene expression
GTP Phosphohydrolases - metabolism
Imports
Inhibition
Messenger RNA
Metabolism
Molecular and cellular biology
Molecular genetics
Nuclear Envelope - metabolism
Nuclear Proteins - metabolism
Oocytes
Physiological aspects
Proteins
Proteins - metabolism
ran GTP-Binding Protein
Ribonucleic acid
RNA
RNA - metabolism
RNA Caps
RNA Precursors - metabolism
RNA, Messenger - metabolism
RNA, Ribosomal - metabolism
RNA, Small Nuclear - metabolism
RNA, Transfer, Amino Acid-Specific - metabolism
Small nuclear RNA
Stomatitis
Transcripts (Written Records)
Transfer RNA
Vesicular stomatitis Indiana virus
vesicular stomatitis virus
Viral Matrix Proteins - physiology
Virus diseases
Viruses
Wheat Germ Agglutinins - pharmacology
Xenopus laevis
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Summary:Transport of macromolecules into and out of nuclei, essential steps in gene expression, are potential points of control. The matrix protein (M protein) of vesicular stomatitis virus (VSV) was shown to block transport of RNAs and proteins between the nucleus and cytoplasm of Xenopus laevis oocytes. The pattern of inhibition indicated that M protein interfered with transport that is dependent on the $\underline{ra}$s-like nuclear guanosine triphosphatase (GTPase) Ran-TC4 and its associated factors. This inhibition of nuclear transport by M protein explains several observations about the effects of VSV infection on host cell gene expression and suggests that RNA export is closely coupled to protein import.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.276.5320.1845