Detection of Neospora sp. from infected bovine tissues by PCR and probe hybridization

Neospora sp. can cause fetal abortion or neurological disease in congenitally infected calves. Latent tissue stages in infected cows may contribute to vertical transmission of Neospora sp. from dam to offspring in multiple pregnancies. In this investigation, the polymerase chain reaction (PCR) and N...

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Bibliographic Details
Published in:The Journal of parasitology 1997-06, Vol.83 (3), p.508-514
Main Authors: Ho, M.S.Y, Barr, B.C, Rowe, J.D, Anderson, M.L, Sverlow, K.W, Packham, A, Marsh, A.E, Conrad, P.A
Format: Article
Language:English
Subjects:
Abortion, Veterinary - parasitology
Animal protozoal diseases
Animals
Biological and medical sciences
Blotting, Southern - veterinary
BOVINAE
Cattle
Cattle Diseases - parasitology
Coccidiosis - parasitology
Coccidiosis - veterinary
DNA, Protozoan - analysis
Female
Infectious diseases
Male
Medical sciences
NEOSPORA
Neospora - genetics
Neospora - isolation & purification
Parasitic diseases
PCR
POLYMERASE CHAIN REACTION
Polymerase Chain Reaction - veterinary
Pregnancy
Pregnancy Complications, Parasitic - parasitology
Pregnancy Complications, Parasitic - veterinary
Protozoal diseases
RNA, Protozoan - genetics
RNA, Ribosomal - genetics
RNA, Small Nuclear - genetics
Sensitivity and Specificity
Therapeutics-Diagnostics
Citations: Items that cite this one
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Summary:Neospora sp. can cause fetal abortion or neurological disease in congenitally infected calves. Latent tissue stages in infected cows may contribute to vertical transmission of Neospora sp. from dam to offspring in multiple pregnancies. In this investigation, the polymerase chain reaction (PCR) and Neospora-specific assay were employed to detect Neospora sp. by amplification of nuclear small-subunit rRNA gene sequences in infected cattle tissues. Tissues from 11 cattle, including 6 experimentally and 2 naturally infected cows, 1 naturally infected newborn calf, and 2 uninfected control cows, were evaluated in this study. Neospora-specific PCR products were amplified from DNAs of different bovine tissues, including brain, spinal cord, heart, lung, kidney, diaphragm, skeletal muscle, and placenta, as well as amniotic fluid samples of infected cattle. The PCR-based amplification and probe hybridization system proved useful in assessing the location of tissue-stage parasites in naturally and experimentally infected cattle, even when Neospora sp. antibody titers fall below normal cut-off values by an indirect immunofluorescent antibody test
ISSN:0022-3395
1937-2345
DOI:10.2307/3284418