Suppressive effects of destruxin B on hepatitis B virus surface antigen gene expression in human hepatoma cells

Destruxin B, a cyclodepsipeptide was originally identified as a plant pathogen from the fungus, Alternaria brassicae. We examined the antiviral activity of destruxin B and found that it suppresses the expression of the hepatitis B viral surface antigen (HBsAg) gene in human hepatoma Hep3B cells whic...

Full description

Saved in:
Bibliographic Details
Published in:Antiviral research 1997-05, Vol.34 (3), p.137-144
Main Authors: Chen, Hua-Chien, Chou, Chen-Kung, Sun, Chang-Ming, Farn Yeh, Sheau
Format: Article
Language:English
Subjects:
Alternaria brassicae
Antiviral agent
Antiviral Agents - pharmacology
Biological and medical sciences
Carcinoma, Hepatocellular
Cyclodepsipeptide
Depsipeptides
Fungus metabolite
Gene Expression Regulation, Viral - drug effects
Gene regulation
General pharmacology
Genes, Viral - drug effects
Hepatitis B Surface Antigens - biosynthesis
Hepatitis B Surface Antigens - genetics
hepatitis B virus
Hepatitis B virus - drug effects
Hepatitis B virus - genetics
Hepatitis B virus - immunology
Humans
Medical sciences
Peptides, Cyclic - pharmacology
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Viral - genetics
RNA, Viral - metabolism
Transfection
Tumor Cells, Cultured
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Destruxin B, a cyclodepsipeptide was originally identified as a plant pathogen from the fungus, Alternaria brassicae. We examined the antiviral activity of destruxin B and found that it suppresses the expression of the hepatitis B viral surface antigen (HBsAg) gene in human hepatoma Hep3B cells which carry an integrated viral gene in its chromosome. In contrast, destruxin B shows no cytotoxic effect on the viability of the cells. Furthermore, it can be shown that destruxin B can reversibly suppress HBsAg production by Hep3B cells in a concentration-dependent manner with EC 50 of 0.5 μM. Northern blot analysis indicates that the suppression of HBsAg gene expression by destruxin B is mainly at the mRNA level. Destruxin B not only suppresses the endogenously expressed HBsAg in the Hep3B cells but also suppresses the HBsAg produced either from the stable transfected HBV DNA in another human hepatoma HuH-7 cell line which carry no endogenous HBV genome. These results suggest that destruxin B may have future potential for development as a specific anti-HBV drug.
ISSN:0166-3542
1872-9096
DOI:10.1016/S0166-3542(97)01031-0