bcl-2 EXPRESSION IN PLEURAL AND EXTRAPLEURAL SOLITARY FIBROUS TUMOURS

• Published in: The Journal of pathology 1997-04, Vol.181 (4), p.362-367
• Main Authors: CHILOSI, MARCO, FACCHETTI, FABIO, DEI TOS, ANGELO P., LESTANI, MAURIZIO, MORASSI, MARIA L., MARTIGNONI, GUIDO, SORIO, CLAUDIO, BENEDETTI, ALICE, MORELLI, LUCA, DOGLIONI, CLAUDIO, BARBERIS, MASSIMO, MENESTRINA, FABIO, VIALE, GIUSEPPE
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Antigens, CD34 - metabolism; bcl-2; Biological and medical sciences; Biomarkers, Tumor - metabolism; Blotting, Western; CD34; Diagnosis, Differential; Female; Humans; Immunoenzyme Techniques; Male; Medical sciences; mesothelioma; Mesothelioma - diagnosis; Mesothelioma - metabolism; Middle Aged; Neoplasm Proteins - metabolism; Neoplasms, Fibrous Tissue - diagnosis; Neoplasms, Fibrous Tissue - metabolism; Pleural Neoplasms - diagnosis; Pleural Neoplasms - metabolism; Pneumology; Proto-Oncogene Proteins c-bcl-2 - metabolism; Soft Tissue Neoplasms - diagnosis; Soft Tissue Neoplasms - metabolism; solitary fibrous tumour; Tumors of the respiratory system and mediastinum
• ISSN: 0022-3417; 1096-9896
• DOI: 10.1002/(SICI)1096-9896(199704)181:4<362::AID-PATH764>3.0.CO;2-Y

This study evaluated the immunoreactivity for bcl‐2, a molecule involved in the control of programmed cell death, in cases of pleural (14) and extrapleural (2) solitary fibrous tumour (SFT), malignant mesotheliomas of different histological types, and a variety of extrapleural CD34‐positive and CD34‐negative spindle‐cell tumours. In all SFTs, strong and diffuse immunostaining was demonstrated with anti‐bcl‐2 antibody, sharply contrasting with the complete lack of staining observed in all mesotheliomas. The specificity of immunodetection of bcl‐2 in SFT was confirmed by immunoblot analysis, showing a band consistent with the bcl‐2 protein. At extrapleural locations, strong bcl‐2 immunoreactivity was observed in Schwannoma (2/3 cases), synovial sarcoma (4/4 cases), and all cases of CD34‐positive gastrointestinal stromal tumour (GIST; 10/10 cases). Most sarcomas were bcl‐2‐negative. Lack of bcl‐2 expression was demonstrated in tumours which can pose problems in the differential diagnosis of SFT and can exhibit haemangiopericytoma‐like features, including haemangiopericytoma (3 cases), dermatofibrosarcoma protuberans (16 cases), and deep‐seated fibrous histiocytoma (3 cases). The constitutive expression of bcl‐2 in SFT widens the spectrum of available markers for these tumours, providing a useful adjunct to their differential diagnosis in difficult cases at pleural and extrapleural sites, and contributing to the understanding of their histogenesis and molecular pathogenesis. © 1997 by John Wiley & Sons, Ltd.