Chemiluminescence in a macrophage cell line modulated by biological response modifiers

• Published in: Journal of leukocyte biology 1989-08, Vol.46 (2), p.103-108
• Main Authors: Tosk, Jeff, Lau, Benjamin H.S., Lui, Paul, Myers, R. Craig, Torrey, Robert R.
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic - pharmacology; Animal cells; Animals; Bacterial Vaccines - pharmacology; BCG; BCG Vaccine - pharmacology; Biological and medical sciences; Biotechnology; Cell Line; Colony-Forming Units Assay; Corynebacterlum parvum; Establishment of new cell lines, improvement of cultural methods, mass cultures; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunobiology; J774 cells; lipopolysaccharide; Lipopolysaccharides - pharmacology; Luminescent Measurements; luminol; Macrophage Activation; Macrophages - cytology; Macrophages - drug effects; Macrophages - physiology; Methods. Procedures. Technologies; Mice; Myeloid cells: ontogeny, maturation, markers, receptors; Oxidation-Reduction; phagocytes; Propionibacterium acnes - immunology; Zymosan - pharmacology
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/jlb.46.2.103

We have studied a murine macrophage cell line, J774, and found these cells capable of a zymosan‐triggered chemiluminescent oxidative burst. Such activity was enhanced by preincubation with Corynebacterium parvum (CP), bacillus Calmette‐Guerin, and Iipopolysaccharide (LPS). Under similar conditions, CP and LPS were shown to enhance J774‐mediated tumor cell lysis. We have also demonstrated that murine interferon α + β rendered J774 cells more sensitive to the actions of CP and LPS. These results indicate that J774 cells may be useful for the in vitro evaluation of biological response modifiers as well as the study of oxygen radical production by macrophages.