Molecular cloning and expression of a bovine endothelial inward rectifier potassium channel

A 5.1 kb cDNA encoding an inward rectifier K + channel (BIK) was isolated from a bovine aortic endothelial cell library. The cDNA codes for a 427-amino-acid protein with two putative transmembrane regions. Sequence analysis reveals that BIK is a member of the Kir2.1 family of inward rectifier K + ch...

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Bibliographic Details
Published in:FEBS letters 1997-06, Vol.409 (2), p.277-282
Main Authors: Forsyth, Scott E, Hoger, Anne, Hoger, Jeff H
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Anti-Arrhythmia Agents - pharmacology
Aorta
Base Sequence
Blotting, Northern
Cattle
cDNA cloning
Cloning, Molecular
Endothelial cell
Endothelium, Vascular - chemistry
Endothelium, Vascular - metabolism
Endothelium, Vascular - physiology
Gene expression
Inward rectifier
K + channel
Membrane Potentials - drug effects
Molecular Sequence Data
Potassium Channels - biosynthesis
Potassium Channels - chemistry
Potassium Channels - genetics
Potassium Channels, Inwardly Rectifying
Rheology
Shear stress
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Summary:A 5.1 kb cDNA encoding an inward rectifier K + channel (BIK) was isolated from a bovine aortic endothelial cell library. The cDNA codes for a 427-amino-acid protein with two putative transmembrane regions. Sequence analysis reveals that BIK is a member of the Kir2.1 family of inward rectifier K + channels. Expression in Xenopus oocytes showed that BIK is a K +-specific strong inward rectifier channel that is sensitive to extracellular Ba 2+, Cs +, and a variety of anti-arrhythmic agents. Northern analysis revealed that endothelial cells express a 5.5 kb BIK mRNA that is sensitive to shear stress.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(97)00514-0