Partial characterization of the gene coding for subunit IV of soybean mitochondrial NADH dehydrogenase

By using the spinach chloroplast atpE gene (epsilon-subunit coding gene) as a probe we have isolated, from a soybean mitochondrial DNA library, a sequence containing a 405 base-pairs (bp) open-reading frame (ORF). This ORF, which is unique in the soybean mitochondrial genome, is probably part of an...

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Bibliographic Details
Published in:Current genetics 1989-02, Vol.15 (2), p.155-160
Main Authors: Wintz, H, Chen, H.C, Pillay, D.T.N
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
DNA, Mitochondrial - genetics
DNA, Mitochondrial - isolation & purification
Exons
Fundamental and applied biological sciences. Psychology
Genes
Genes. Genome
Glycine max
Humans
Introns
Macromolecular Substances
mitochondria
Mitochondria - enzymology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
NADH dehydrogenase
nucleotide sequences
Plants - enzymology
Plants - genetics
Restriction Mapping
Sequence Homology, Nucleic Acid
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Summary:By using the spinach chloroplast atpE gene (epsilon-subunit coding gene) as a probe we have isolated, from a soybean mitochondrial DNA library, a sequence containing a 405 base-pairs (bp) open-reading frame (ORF). This ORF, which is unique in the soybean mitochondrial genome, is probably part of an exon of the gene coding for subunit IV of the NADH dehydrogenase complex. The predicted protein shows 42% sequence similarity with the C-terminal region the Aspergillus nidulans NAD4 protein. The gene is split by a class II intron which is larger than 1,950 bp. Transcription analysis revealed a single 2,200 nucleotide long transcript which does not contain the intron sequence.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00435463