Changes in the starvation response through covalent cell attachment

Covalent attachment of Candida utilis cells, possibly simulating natural microbial immobilizations, stimulated stable and significant enhancement of extracellular production of alkaline protease, specifically induced by four different starvation conditions. The enzyme analysis confirmed the identity...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 1997-05, Vol.71 (4), p.369-373
Main Author: JIRKU, V
Format: Article
Language:English
Subjects:
Action of physical and chemical agents
beta-Alanine - analogs & derivatives
beta-Alanine - chemistry
Biological and medical sciences
Biotechnology
Candida - chemistry
Candida - enzymology
Candida - ultrastructure
Candida utilis
Cell Adhesion
Endopeptidases - metabolism
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Methacrylates - chemistry
Methods. Procedures. Technologies
Microbiology
Miscellaneous
Mycology
Proteins
Starvation
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Summary:Covalent attachment of Candida utilis cells, possibly simulating natural microbial immobilizations, stimulated stable and significant enhancement of extracellular production of alkaline protease, specifically induced by four different starvation conditions. The enzyme analysis confirmed the identity of the proteases released under all conditions of starvation and no parallel production of other proteolytic enzyme. The enhancement phenomenon as a uniform and stable effect of the whole cell immobilization is discussed in relation to the effect of multipoint, cell-solid surface contact, potentially bringing positive modulations of complex, cellular functions.
ISSN:0003-6072
1572-9699
DOI:10.1023/A:1000235610299