Metabotropic GABA receptors regulate acetylcholine responses on snail neurons

1. 1. The A type of acetylcholine response of Helix neurons is downmodulated by low concentrations of GABA that do not elicit any measurable change in membrane potential or conductance. 2. 2. We find that these physiological actions are associated with an increase in both intracellular cyclic AMP le...

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Bibliographic Details
Published in:General pharmacology 1997-07, Vol.29 (1), p.67-72
Main Authors: Azatian, K.V., Ayrapetyan, S.N., Carpenter, D.O.
Format: Article
Language:English
Subjects:
Acetylcholine - pharmacology
Animals
Biochemistry. Physiology. Immunology
Biological and medical sciences
Calcium - metabolism
calcium Na/Ca exchange
cyclic AMP
Cyclic AMP - metabolism
Fundamental and applied biological sciences. Psychology
Helix (Snails) - physiology
Invertebrates
Mollusca
Neurons - physiology
ouabain
Patch-Clamp Techniques
Physiology. Development
Protein kinase A
Receptors, GABA - physiology
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Summary:1. 1. The A type of acetylcholine response of Helix neurons is downmodulated by low concentrations of GABA that do not elicit any measurable change in membrane potential or conductance. 2. 2. We find that these physiological actions are associated with an increase in both intracellular cyclic AMP levels and 45Ca 2+ influx. 3. 3. The modulation of the acetylcholine response by GABA is blocked when the neurons are injected with EGTA to prevent a rise in intracellular Ca 2+ concentration or when tolbutamide, an inhibitor of protein kinase A, is applied. 4. 4. These results are consistent with the effects of GABA being mediated by a metabotropic GABA receptor that is activated at very low GABA concentrations and mediates modulation of the acetylcholine response via regulation of intracellular Ca 2+ and cyclic AMP levels.
ISSN:0306-3623
1879-0011
DOI:10.1016/S0306-3623(96)00568-X