Acute Experimental Distal Colitis Alters Colonic Transit in Rats

• Published in: The Journal of surgical research 1997-04, Vol.69 (1), p.107-112
• Main Authors: Myers, Brian S., Dempsey, Daniel T., Yasar, Saban, Martin, John S., Parkman, Henry P., Ryan, James P.
• Format: Article
• Language: English
• Subjects: Acetylcholine - pharmacology; Acute Disease; Animals; Biological and medical sciences; Colitis - physiopathology; Colon - drug effects; Colon - physiopathology; Gastroenterology. Liver. Pancreas. Abdomen; Gastrointestinal Motility; Gastrointestinal Transit; Male; Medical sciences; Muscle, Smooth - drug effects; Other diseases. Semiology; Potassium Chloride - pharmacology; Rats; Rats, Sprague-Dawley; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Time Factors
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1006/jsre.1997.5042

Data from humans with active distal colitis suggest that the proximal colon exhibits increased contractile activity and delayed transit, whereas the distal colon shows decreased contractile activity and rapid transit. The present study used the acetic acid rat model of experimental colitis to determine the effect of distal colitis on total and regional colonic transitin vivoand on thein vitrocontractility of circular smooth muscle from the proximal and distal colon. Distal colitis was induced in rats by intracolonic administration of 4% acetic acid; sham control rats received saline enemas. Control and colitic rats were studied 2 days postenemas. Total colon transit was determined by calculating the geometric center of distribution of a radiolabeled marker (51Cr) instilled into the proximal colon. Regional transit was assessed by expressing the radioactivity in the cecum, proximal and distal colon, and excreted stool as a percent of total radioactivity. Muscle strips from the proximal and distal colon were stimulated with 100 μMacetylcholine (ACh) and 60 mMKCl and the tension was expressed as kilograms per square centimeter. Distal colitis was characterized by decreased total colon transit, increased retention of marker in the cecum and proximal colon, and decreased retention of marker in the distal colon.In vitrocontractility studies revealed that distal colitis increased proximal colon circular smooth muscle contractility and decreased distal colon circular smooth muscle contractility to both ACh and potassium. Distal colitis is associated with regional differences in colonic circular smooth muscle contractility, which may contribute to delayed transit in the proximal colon and rapid transit in the distal colon.