The Y chromosome of the mouse is decondensed in Sertoli cells

The condensation of the Y chromosome in mouse cells was studied with two repetitive DNA probes, pY353/B and M34. Both DNA probes are specific to the Y chromosome and hybridize in situ along the whole chromosome. Due to the high resolution of the in situ hybridization technique with non-radioactive l...

Full description

Saved in:
Bibliographic Details
Published in:Chromosoma 1989-05, Vol.97 (6), p.429-433
Main Authors: GUTTENBACH, M, SCHMID, M, JAUCH, A, VOGT, P
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cytogenetics
DNA Probes
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Interphase
Male
Mice
Mice, Inbred Strains
Nucleic Acid Hybridization
Sertoli Cells - metabolism
Sertoli Cells - ultrastructure
Spermatogenesis
Transcription, Genetic
Vertebrata
Y Chromosome - metabolism
Y Chromosome - ultrastructure
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The condensation of the Y chromosome in mouse cells was studied with two repetitive DNA probes, pY353/B and M34. Both DNA probes are specific to the Y chromosome and hybridize in situ along the whole chromosome. Due to the high resolution of the in situ hybridization technique with non-radioactive labeled DNA probes it was possible to observe the degree of condensation of the Y chromosome in the interphase cell nuclei of various somatic tissues and on testes preparations. The Sertoli cells were the only cell type in which the Y chromosome was always observed to be in a highly decondensed state. The decondensation of the Y chromosome in the Sertoli cells supports the view that the genetic activity of the Y chromosome is cell autonomous and opens the way to its molecular analysis.
ISSN:0009-5915
1432-0886
DOI:10.1007/BF00295026