High-performance liquid chromatographic determination of myocardial interstitial dihydroxyphenylglycol

This study describes a high-performance liquid chromatographic method with electrochemical detection (HPLC–ED) for monitoring dihydroxyphenylglycol (DHPG) in the myocardial interstitial space. Using a cardiac dialysis technique, 10-μl dialysates were sampled from the myocardial interstitial space (1...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1997-05, Vol.693 (1), p.218-221
Main Authors: Takauchi, Yuji, Kitagawa, Hirotoshi, Kawada, Toru, Akiyama, Tsuyoshi, Yamazaki, Toji
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cats
Cell structures and functions
Chromatography, High Pressure Liquid
Dialysis
Dihydroxyphenylglycol
Electrochemistry
Extracellular Space - chemistry
Female
Fundamental and applied biological sciences. Psychology
Male
Methoxyhydroxyphenylglycol - analogs & derivatives
Methoxyhydroxyphenylglycol - analysis
Mitochondria and cell respiration
Molecular and cellular biology
Myocardium - chemistry
Reproducibility of Results
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Summary:This study describes a high-performance liquid chromatographic method with electrochemical detection (HPLC–ED) for monitoring dihydroxyphenylglycol (DHPG) in the myocardial interstitial space. Using a cardiac dialysis technique, 10-μl dialysates were sampled from the myocardial interstitial space (1-min fractions) and were injected directly into the HPLC–ED system. The in vitro quantification limit for DHPG was 250 fg in a 10-μl injection. The basal DHPG concentration of dialysate was 181±46 pg/ml. This system offers a new possibility for monitoring myocardial interstitial DHPG levels.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(97)00003-0