Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization

• Published in: Biology of reproduction 1997-07, Vol.57 (1), p.49-53
• Main Authors: Funahashi, H, Cantley, T.C, Day, B.N
• Format: Article
• Language: English
• Subjects: AMP; Animal productions; Animals; Biological and medical sciences; Blastocyst - drug effects; Bucladesine - pharmacology; C-AMP; CERDO; CUMULUS OOCYTE COMPLEXES; CUMULUS OOPHORUS; DESARROLLO EMBRIONARIO; DEVELOPPEMENT EMBRYONNAIRE; EMBRYO TRANSFER; Embryonic and Fetal Development - drug effects; EMBRYONIC DEVELOPMENT; EXPERIMENTACION IN VITRO; EXPERIMENTATION IN VITRO; FECONDATION; FECUNDACION; Female; FERTILIZATION; Fertilization in Vitro; Fundamental and applied biological sciences. Psychology; GAMETE; GAMETES; GAMETOS; GERMINAL VESICLE; IN VITRO EXPERIMENTATION; IN VITRO MATURATION; In Vitro Techniques; MADURACION; Male; MATURATION; MEIOSE; MEIOSIS; Meiosis - drug effects; MEIOTIC SYNCHRONIZATION; Oocytes - cytology; Oocytes - drug effects; Oocytes - growth & development; OVA; OVULE; OVULO; PORCIN; Pregnancy; SINCRONIZACION; SWINE; Swine - embryology; Swine - physiology; SYNCHRONISATION; SYNCHRONIZATION; Terrestrial animal productions; TRANSFERENCIA DE EMBRIONES; TRANSFERT EMBRYONNAIRE; Vertebrates
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1095/biolreprod57.1.49

The effect of stage of maturation of the germinal vesicle of porcine oocytes at the time of in vitro maturation on subsequent developmental competence was examined. A large variation exists in the germinal vesicle morphology of oocytes at the time of collection of cumulus-oocyte complexes (COCs) and after culture in the absence of dibutyryl cAMP (dbcAMP) for 20 h. However, the morphology of the germinal vesicle was synchronized to a specific stage after culture in the presence of 1 mM dbcAMP for 20 h. There was no difference in germinal vesicle breakdown rate (total mean, 75.0 +/- 5.4%) or in maturation rate (total mean, 82.1 +/- 2.1 %) at 28 and 44 h of culture, respectively. However, differences in meiotic progress of oocytes were observed (p < 0.05) at 36 h of culture when COCs were exposed to dbcAMP for the first 20 h of maturation, as compared to controls. The incidence of embryos that developed to the blastocyst stage after in vitro fertilization was higher (p < 0.05) when COCs were exposed to dbcAMP (21.5 +/- 2.5%) as compared to controls (9.2 +/- 1.6%). After transfer of experimental embryos to four recipient gilts, the three pregnant recipients delivered 19 live piglets. These results indicate that exposure of COCs to dbcAMP for the first 20 h of culture for maturation increases the homogeneity of oocyte nuclear maturation and improves the efficiency of in vitro production of swine embryos.