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Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization
The effect of stage of maturation of the germinal vesicle of porcine oocytes at the time of in vitro maturation on subsequent developmental competence was examined. A large variation exists in the germinal vesicle morphology of oocytes at the time of collection of cumulus-oocyte complexes (COCs) and...
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| Published in: | Biology of reproduction 1997-07, Vol.57 (1), p.49-53 |
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| Language: | English |
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| container_end_page | 53 |
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| container_title | Biology of reproduction |
| container_volume | 57 |
| creator | Funahashi, H Cantley, T.C Day, B.N |
| description | The effect of stage of maturation of the germinal vesicle of porcine oocytes at the time of in vitro maturation on subsequent
developmental competence was examined. A large variation exists in the germinal vesicle morphology of oocytes at the time
of collection of cumulus-oocyte complexes (COCs) and after culture in the absence of dibutyryl cAMP (dbcAMP) for 20 h. However,
the morphology of the germinal vesicle was synchronized to a specific stage after culture in the presence of 1 mM dbcAMP for
20 h. There was no difference in germinal vesicle breakdown rate (total mean, 75.0 +/- 5.4%) or in maturation rate (total
mean, 82.1 +/- 2.1 %) at 28 and 44 h of culture, respectively. However, differences in meiotic progress of oocytes were observed
(p < 0.05) at 36 h of culture when COCs were exposed to dbcAMP for the first 20 h of maturation, as compared to controls.
The incidence of embryos that developed to the blastocyst stage after in vitro fertilization was higher (p < 0.05) when COCs
were exposed to dbcAMP (21.5 +/- 2.5%) as compared to controls (9.2 +/- 1.6%). After transfer of experimental embryos to four
recipient gilts, the three pregnant recipients delivered 19 live piglets. These results indicate that exposure of COCs to
dbcAMP for the first 20 h of culture for maturation increases the homogeneity of oocyte nuclear maturation and improves the
efficiency of in vitro production of swine embryos. |
| doi_str_mv | 10.1095/biolreprod57.1.49 |
| format | article |
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developmental competence was examined. A large variation exists in the germinal vesicle morphology of oocytes at the time
of collection of cumulus-oocyte complexes (COCs) and after culture in the absence of dibutyryl cAMP (dbcAMP) for 20 h. However,
the morphology of the germinal vesicle was synchronized to a specific stage after culture in the presence of 1 mM dbcAMP for
20 h. There was no difference in germinal vesicle breakdown rate (total mean, 75.0 +/- 5.4%) or in maturation rate (total
mean, 82.1 +/- 2.1 %) at 28 and 44 h of culture, respectively. However, differences in meiotic progress of oocytes were observed
(p < 0.05) at 36 h of culture when COCs were exposed to dbcAMP for the first 20 h of maturation, as compared to controls.
The incidence of embryos that developed to the blastocyst stage after in vitro fertilization was higher (p < 0.05) when COCs
were exposed to dbcAMP (21.5 +/- 2.5%) as compared to controls (9.2 +/- 1.6%). After transfer of experimental embryos to four
recipient gilts, the three pregnant recipients delivered 19 live piglets. These results indicate that exposure of COCs to
dbcAMP for the first 20 h of culture for maturation increases the homogeneity of oocyte nuclear maturation and improves the
efficiency of in vitro production of swine embryos.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod57.1.49</identifier><identifier>PMID: 9209079</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>AMP ; Animal productions ; Animals ; Biological and medical sciences ; Blastocyst - drug effects ; Bucladesine - pharmacology ; C-AMP ; CERDO ; CUMULUS OOCYTE COMPLEXES ; CUMULUS OOPHORUS ; DESARROLLO EMBRIONARIO ; DEVELOPPEMENT EMBRYONNAIRE ; EMBRYO TRANSFER ; Embryonic and Fetal Development - drug effects ; EMBRYONIC DEVELOPMENT ; EXPERIMENTACION IN VITRO ; EXPERIMENTATION IN VITRO ; FECONDATION ; FECUNDACION ; Female ; FERTILIZATION ; Fertilization in Vitro ; Fundamental and applied biological sciences. Psychology ; GAMETE ; GAMETES ; GAMETOS ; GERMINAL VESICLE ; IN VITRO EXPERIMENTATION ; IN VITRO MATURATION ; In Vitro Techniques ; MADURACION ; Male ; MATURATION ; MEIOSE ; MEIOSIS ; Meiosis - drug effects ; MEIOTIC SYNCHRONIZATION ; Oocytes - cytology ; Oocytes - drug effects ; Oocytes - growth & development ; OVA ; OVULE ; OVULO ; PORCIN ; Pregnancy ; SINCRONIZACION ; SWINE ; Swine - embryology ; Swine - physiology ; SYNCHRONISATION ; SYNCHRONIZATION ; Terrestrial animal productions ; TRANSFERENCIA DE EMBRIONES ; TRANSFERT EMBRYONNAIRE ; Vertebrates</subject><ispartof>Biology of reproduction, 1997-07, Vol.57 (1), p.49-53</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2763665$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9209079$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Funahashi, H</creatorcontrib><creatorcontrib>Cantley, T.C</creatorcontrib><creatorcontrib>Day, B.N</creatorcontrib><title>Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The effect of stage of maturation of the germinal vesicle of porcine oocytes at the time of in vitro maturation on subsequent
developmental competence was examined. A large variation exists in the germinal vesicle morphology of oocytes at the time
of collection of cumulus-oocyte complexes (COCs) and after culture in the absence of dibutyryl cAMP (dbcAMP) for 20 h. However,
the morphology of the germinal vesicle was synchronized to a specific stage after culture in the presence of 1 mM dbcAMP for
20 h. There was no difference in germinal vesicle breakdown rate (total mean, 75.0 +/- 5.4%) or in maturation rate (total
mean, 82.1 +/- 2.1 %) at 28 and 44 h of culture, respectively. However, differences in meiotic progress of oocytes were observed
(p < 0.05) at 36 h of culture when COCs were exposed to dbcAMP for the first 20 h of maturation, as compared to controls.
The incidence of embryos that developed to the blastocyst stage after in vitro fertilization was higher (p < 0.05) when COCs
were exposed to dbcAMP (21.5 +/- 2.5%) as compared to controls (9.2 +/- 1.6%). After transfer of experimental embryos to four
recipient gilts, the three pregnant recipients delivered 19 live piglets. These results indicate that exposure of COCs to
dbcAMP for the first 20 h of culture for maturation increases the homogeneity of oocyte nuclear maturation and improves the
efficiency of in vitro production of swine embryos.</description><subject>AMP</subject><subject>Animal productions</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - drug effects</subject><subject>Bucladesine - pharmacology</subject><subject>C-AMP</subject><subject>CERDO</subject><subject>CUMULUS OOCYTE COMPLEXES</subject><subject>CUMULUS OOPHORUS</subject><subject>DESARROLLO EMBRIONARIO</subject><subject>DEVELOPPEMENT EMBRYONNAIRE</subject><subject>EMBRYO TRANSFER</subject><subject>Embryonic and Fetal Development - drug effects</subject><subject>EMBRYONIC DEVELOPMENT</subject><subject>EXPERIMENTACION IN VITRO</subject><subject>EXPERIMENTATION IN VITRO</subject><subject>FECONDATION</subject><subject>FECUNDACION</subject><subject>Female</subject><subject>FERTILIZATION</subject><subject>Fertilization in Vitro</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GAMETE</subject><subject>GAMETES</subject><subject>GAMETOS</subject><subject>GERMINAL VESICLE</subject><subject>IN VITRO EXPERIMENTATION</subject><subject>IN VITRO MATURATION</subject><subject>In Vitro Techniques</subject><subject>MADURACION</subject><subject>Male</subject><subject>MATURATION</subject><subject>MEIOSE</subject><subject>MEIOSIS</subject><subject>Meiosis - drug effects</subject><subject>MEIOTIC SYNCHRONIZATION</subject><subject>Oocytes - cytology</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - growth & development</subject><subject>OVA</subject><subject>OVULE</subject><subject>OVULO</subject><subject>PORCIN</subject><subject>Pregnancy</subject><subject>SINCRONIZACION</subject><subject>SWINE</subject><subject>Swine - embryology</subject><subject>Swine - physiology</subject><subject>SYNCHRONISATION</subject><subject>SYNCHRONIZATION</subject><subject>Terrestrial animal productions</subject><subject>TRANSFERENCIA DE EMBRIONES</subject><subject>TRANSFERT EMBRYONNAIRE</subject><subject>Vertebrates</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkU1v1DAQhiMEKkvhB3BA8gFx28UfiR0fUVU-pEocSs-R44x3jRxPsL1dwi_iZ-KKFT1y8Uie533n1UzTvGZ0x6ju3o8eQ4Il4dSpHdu1-kmzYR3XW8Vl_7TZUErlVggpnjcvcv5OKWsFFxfNheZUU6U3ze_bNdpDwuh_meIxEnRkBo_ZZ-IjWTBZH4Eg2rVAJuNK4OeC-ZiAFCSTH49lTWsgdrXBW2ImiFVbFTNGXA6Yl4MpQPxcQ95XgwnuIeAyQyymqnBeoEC0QByGgCcf9w9j731JSByk4sM52MvmmTMhw6tzvWzuPl5_u_q8vfn66cvVh5utE0yVbS-d4Lp3bmRj27e6072GnkrXCcuFevioqzHGCKcN167VspNTO7X9yJmGSVw27_761sA_jpDLMPtsIQQTAY95UJpqrbj6L8ikEJzKtoJvzuBxnGEaluRnk9bhfIPaf3vum2xNcMlE6_M_jCsppOwesYPfH04-wZBnE0I1FcPpdOrUwIZWP05zBgezT9Xp7pbVyLRT9RV_AFfxstA</recordid><startdate>19970701</startdate><enddate>19970701</enddate><creator>Funahashi, H</creator><creator>Cantley, T.C</creator><creator>Day, B.N</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19970701</creationdate><title>Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization</title><author>Funahashi, H ; Cantley, T.C ; Day, B.N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f317t-86f3298ffb1b48495989e806f53c2374959268aaa3f9a29f49656d4d48b219ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>AMP</topic><topic>Animal productions</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - drug effects</topic><topic>Bucladesine - pharmacology</topic><topic>C-AMP</topic><topic>CERDO</topic><topic>CUMULUS OOCYTE COMPLEXES</topic><topic>CUMULUS OOPHORUS</topic><topic>DESARROLLO EMBRIONARIO</topic><topic>DEVELOPPEMENT EMBRYONNAIRE</topic><topic>EMBRYO TRANSFER</topic><topic>Embryonic and Fetal Development - drug effects</topic><topic>EMBRYONIC DEVELOPMENT</topic><topic>EXPERIMENTACION IN VITRO</topic><topic>EXPERIMENTATION IN VITRO</topic><topic>FECONDATION</topic><topic>FECUNDACION</topic><topic>Female</topic><topic>FERTILIZATION</topic><topic>Fertilization in Vitro</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GAMETE</topic><topic>GAMETES</topic><topic>GAMETOS</topic><topic>GERMINAL VESICLE</topic><topic>IN VITRO EXPERIMENTATION</topic><topic>IN VITRO MATURATION</topic><topic>In Vitro Techniques</topic><topic>MADURACION</topic><topic>Male</topic><topic>MATURATION</topic><topic>MEIOSE</topic><topic>MEIOSIS</topic><topic>Meiosis - drug effects</topic><topic>MEIOTIC SYNCHRONIZATION</topic><topic>Oocytes - cytology</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - growth & development</topic><topic>OVA</topic><topic>OVULE</topic><topic>OVULO</topic><topic>PORCIN</topic><topic>Pregnancy</topic><topic>SINCRONIZACION</topic><topic>SWINE</topic><topic>Swine - embryology</topic><topic>Swine - physiology</topic><topic>SYNCHRONISATION</topic><topic>SYNCHRONIZATION</topic><topic>Terrestrial animal productions</topic><topic>TRANSFERENCIA DE EMBRIONES</topic><topic>TRANSFERT EMBRYONNAIRE</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Funahashi, H</creatorcontrib><creatorcontrib>Cantley, T.C</creatorcontrib><creatorcontrib>Day, B.N</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Funahashi, H</au><au>Cantley, T.C</au><au>Day, B.N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1997-07-01</date><risdate>1997</risdate><volume>57</volume><issue>1</issue><spage>49</spage><epage>53</epage><pages>49-53</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>The effect of stage of maturation of the germinal vesicle of porcine oocytes at the time of in vitro maturation on subsequent
developmental competence was examined. A large variation exists in the germinal vesicle morphology of oocytes at the time
of collection of cumulus-oocyte complexes (COCs) and after culture in the absence of dibutyryl cAMP (dbcAMP) for 20 h. However,
the morphology of the germinal vesicle was synchronized to a specific stage after culture in the presence of 1 mM dbcAMP for
20 h. There was no difference in germinal vesicle breakdown rate (total mean, 75.0 +/- 5.4%) or in maturation rate (total
mean, 82.1 +/- 2.1 %) at 28 and 44 h of culture, respectively. However, differences in meiotic progress of oocytes were observed
(p < 0.05) at 36 h of culture when COCs were exposed to dbcAMP for the first 20 h of maturation, as compared to controls.
The incidence of embryos that developed to the blastocyst stage after in vitro fertilization was higher (p < 0.05) when COCs
were exposed to dbcAMP (21.5 +/- 2.5%) as compared to controls (9.2 +/- 1.6%). After transfer of experimental embryos to four
recipient gilts, the three pregnant recipients delivered 19 live piglets. These results indicate that exposure of COCs to
dbcAMP for the first 20 h of culture for maturation increases the homogeneity of oocyte nuclear maturation and improves the
efficiency of in vitro production of swine embryos.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>9209079</pmid><doi>10.1095/biolreprod57.1.49</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3363 |
| ispartof | Biology of reproduction, 1997-07, Vol.57 (1), p.49-53 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79099727 |
| source | Oxford Journals Online |
| subjects | AMP Animal productions Animals Biological and medical sciences Blastocyst - drug effects Bucladesine - pharmacology C-AMP CERDO CUMULUS OOCYTE COMPLEXES CUMULUS OOPHORUS DESARROLLO EMBRIONARIO DEVELOPPEMENT EMBRYONNAIRE EMBRYO TRANSFER Embryonic and Fetal Development - drug effects EMBRYONIC DEVELOPMENT EXPERIMENTACION IN VITRO EXPERIMENTATION IN VITRO FECONDATION FECUNDACION Female FERTILIZATION Fertilization in Vitro Fundamental and applied biological sciences. Psychology GAMETE GAMETES GAMETOS GERMINAL VESICLE IN VITRO EXPERIMENTATION IN VITRO MATURATION In Vitro Techniques MADURACION Male MATURATION MEIOSE MEIOSIS Meiosis - drug effects MEIOTIC SYNCHRONIZATION Oocytes - cytology Oocytes - drug effects Oocytes - growth & development OVA OVULE OVULO PORCIN Pregnancy SINCRONIZACION SWINE Swine - embryology Swine - physiology SYNCHRONISATION SYNCHRONIZATION Terrestrial animal productions TRANSFERENCIA DE EMBRIONES TRANSFERT EMBRYONNAIRE Vertebrates |
| title | Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization |
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