Confirmation of the Structure of Lipid A Derived from the Lipopolysaccharide of Rhodobacter sphaeroides by a Combination of MALDI, LSIMS, and Tandem Mass Spectrometry

The chemical structure of nontoxic diphosphoryl lipid A from Rhodobacter sphaeroides was confirmed using a combination of LSIMS (on a two-sector mass spectrometer) and MALDI (on time-of-flight and ion trap mass spectrometers) in conjunction with tandem mass spectrometry in both positive and negative...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1997-07, Vol.69 (13), p.2317-2322
Main Authors: Kaltashov, Igor A, Doroshenko, Vladimir, Cotter, Robert J, Takayama, Kuni, Qureshi, Nilofer
Format: Article
Language:English
Subjects:
Bacteria
Bacteriology
Biological and medical sciences
Chemistry
Fundamental and applied biological sciences. Psychology
Lipid A - chemistry
Lipids
Microbiology
Molecular Conformation
Molecular Weight
Morphology, structure, chemical composition
Rhodobacter sphaeroides - chemistry
Scientific imaging
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrometry, Mass, Secondary Ion
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Summary:The chemical structure of nontoxic diphosphoryl lipid A from Rhodobacter sphaeroides was confirmed using a combination of LSIMS (on a two-sector mass spectrometer) and MALDI (on time-of-flight and ion trap mass spectrometers) in conjunction with tandem mass spectrometry in both positive and negative ion modes. Accurate molecular weight measurement accompanied by the analysis of fragment ion masses yielded the composition of fatty acyl groups. Tandem experiments (collisionally induced dissociation of both quasimolecular and oxonium ions) were also performed, revealing the precise location and nature of the fatty acyl groups on the disaccharide backbone.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac9612943