Survival of Lactic Acid Bacteria in a Dynamic Model of the Stomach and Small Intestine: Validation and the Effects of Bile

• Published in: Journal of dairy science 1997-06, Vol.80 (6), p.1031-1037
• Main Authors: Marteau, P., Minekus, M., Havenaar, R., Huis In’t Veld, J.H.J.
• Format: Article
• Language: English
• Subjects: Animals; APPAREIL DIGESTIF; Applied microbiology; BACTERIAS ACIDOLACTICAS; BACTERIE LACTIQUE; BIFIDOBACTERIUM; Bifidobacterium - growth & development; Bifidobacterium - isolation & purification; Bifidobacterium - physiology; BILE; Bile Acids and Salts - pharmacology; BILIS; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Cattle; Dose-Response Relationship, Drug; Female; Fundamental and applied biological sciences. Psychology; gastrointestinal model; Humans; Hydrogen-Ion Concentration; INTESTIN; Intestine, Small - microbiology; Intestine, Small - physiology; INTESTINOS; lactic acid bacteria; Lactobacillus - growth & development; Lactobacillus - isolation & purification; Lactobacillus - physiology; LACTOBACILLUS ACIDOPHILUS; LACTOBACILLUS DELBRUECKII; LAIT FERMENTE; LECHE FERMENTADA; Microbiology; Milk - metabolism; Milk - microbiology; Mission oriented research; MODELE; MODELE DYNAMIQUE; MODELOS; MODELOS DINAMICOS; Models, Biological; Physiology and metabolism; SALES BILIARES; SEL BILIAIRE; SISTEMA DIGESTIVO; Stomach - microbiology; Stomach - physiology; Streptococcus - growth & development; Streptococcus - isolation & purification; Streptococcus - physiology; STREPTOCOCCUS THERMOPHILUS; SUPERVIVENCIA; SURVIE; survival; Time Factors; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); YAOURT; YOGUR
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(97)76027-2

This study was conducted to validate a dynamic model of the stomach and small intestine to quantify the survival of lactic acid bacteria and to assess the influence of gastrointestinal secretions. The survival of a single strain of each of the following species, Bifidobacterium bifidum, Lactobacillus acidophilus, Lactobacillus bulgaricus, and Streptococcus thermophilus, was measured under physiological conditions (e.g., peristalsis, changes in pH, and changes in concentrations of enzymes and bile) and were compared with data obtained from humans. No significant differences were found between the in vitro and in vivo data, indicating that the model has a predictive value for the survival of these bacteria in humans. The survival of these strains of lactic acid bacteria in the gastrointestinal model was investigated under two different conditions in the small intestine: simulation of physiological secretion of bile and low bile secretion. Reductions in viability were significantly different between the bacterial species. The dose-response effect of bile on the survival of the tested bacteria was significant, demonstrating the bactericidal effect of bile salts. This study demonstrates the differences among bacterial species in their sensitivity to gastric and intestinal secretions.