AP-1 mediates trans-synaptic induction of tyrosine hydroxylase gene expression in adrenal medulla but not in superior cervical ganglia

• Published in: Journal of neuroscience research 1997-06, Vol.48 (6), p.489-498
• Main Authors: Trocmé, Caryn, Mallet, Jacques, Biguet, Nicole Faucon
• Format: Article
• Language: English
• Subjects: Adrenal Medulla - drug effects; Adrenal Medulla - embryology; Adrenal Medulla - metabolism; Animals; AP-1 transcription factor; c-Fos; Catecholamines - biosynthesis; Enzyme Induction - drug effects; Macromolecular Substances; Male; Nerve Tissue Proteins - biosynthesis; Nerve Tissue Proteins - genetics; Organ Specificity; Promoter Regions, Genetic; Proto-Oncogene Proteins c-fos - metabolism; Proto-Oncogene Proteins c-jun - metabolism; Rats; Rats, Sprague-Dawley; reserpine; Reserpine - pharmacology; RNA, Messenger - analysis; Signal Transduction; Superior Cervical Ganglion - drug effects; Superior Cervical Ganglion - embryology; Superior Cervical Ganglion - metabolism; Synapses - metabolism; trans-synaptic; Transcription Factor AP-1 - physiology; Tyrosine 3-Monooxygenase - biosynthesis; Tyrosine 3-Monooxygenase - genetics; tyrosine hydroxylase
• ISSN: 0360-4012; 1097-4547
• DOI: 10.1002/(SICI)1097-4547(19970615)48:6<489::AID-JNR1>3.0.CO;2-6

Reserpine treatment leads to a rapid trans‐synaptic increase of the tyrosine hydroxylase (TH) gene transcription rate and mRNA levels in catecholaminergic tissues including the adrenal medulla (AM) and the superior cervical ganglia (SCG). In the AM, the formation of a specific protein complex with the TPA‐responsive element located in the proximal region of the TH gene was enhanced between 30 min and 8 hr following the injection. This complex appears to contain a member of the Fos family and an antigenically related Jun protein. Moreover, the prolonged and enhanced expression of the c‐Fos protein in the AM and its phosphorylation are likely to contribute to the increased TH transcription following reserpine treatment. Most strikingly, in the SCG, the trans‐synaptic induction of TH transcription is transduced by totally different mechanisms, since no AP‐1 complex and only minute amounts of c‐Fos immunoreactivity were detected. Our study provides the first demonstration that, following the same stimulus, the induced expression of a single gene is mediated by different cis‐ and trans‐acting factors in two distinct tissues sharing the same embryonic origin. J. Neurosci. Res. 48:489–498, 1997. © 1997 Wiley‐Liss Inc.