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Transformation of mouse mammary epithelial cells with the Ha- ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production
An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha- ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level...
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| Published in: | FEBS letters 1989-07, Vol.250 (2), p.474-478 |
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| Main Authors: | , , , , , |
| Format: | Article |
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| cites | cdi_FETCH-LOGICAL-c5273-b835119be074b10be32f7edfbb6161b3e5e197b8d86a178f38b98fdb63ba294b3 |
| container_end_page | 478 |
| container_issue | 2 |
| container_start_page | 474 |
| container_title | FEBS letters |
| container_volume | 250 |
| creator | Ciardiello, Fortunato Hynes, Nancy Kim, Nancy Valverius, Eva M. Lippman, Marc E. Salomon, David S. |
| description | An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-
ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21
ras
expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for
ras because
neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression. |
| doi_str_mv | 10.1016/0014-5793(89)80779-3 |
| format | article |
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ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21
ras
expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for
ras because
neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(89)80779-3</identifier><identifier>PMID: 2568949</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Northern ; Cell Line, Transformed ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Cell Transformation, Neoplastic - metabolism ; Cells, Cultured ; CM, conditioned medium ; DMEM, Dulbecco's modified Eagle's medium ; EGF, epidermal growth factor ; Epithelial Cells ; Epithelium - metabolism ; FBS, fetal bovine serum ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Mammary Glands, Animal - cytology ; Mammary Glands, Animal - metabolism ; Mice ; Molecular and cellular biology ; Oncogene ; Oncogene Protein p21(ras) ; Oncogene Proteins, Viral - genetics ; Oncogene, neu ; Oncogene, ras ; Proto-Oncogene Proteins - genetics ; Proto-Oncogenes ; Receptor, ErbB-2 ; RIA, radioimmunoassay ; RNA, Messenger - analysis ; RRA, radioreceptor assay ; TGFα, transforming growth factor-α ; Transformation ; Transforming growth factor-α ; Transforming Growth Factors - biosynthesis ; Viral Proteins - genetics</subject><ispartof>FEBS letters, 1989-07, Vol.250 (2), p.474-478</ispartof><rights>1989</rights><rights>FEBS Letters 250 (1989) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5273-b835119be074b10be32f7edfbb6161b3e5e197b8d86a178f38b98fdb63ba294b3</citedby><cites>FETCH-LOGICAL-c5273-b835119be074b10be32f7edfbb6161b3e5e197b8d86a178f38b98fdb63ba294b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579389807793$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3547,27923,27924,45779</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19298561$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2568949$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ciardiello, Fortunato</creatorcontrib><creatorcontrib>Hynes, Nancy</creatorcontrib><creatorcontrib>Kim, Nancy</creatorcontrib><creatorcontrib>Valverius, Eva M.</creatorcontrib><creatorcontrib>Lippman, Marc E.</creatorcontrib><creatorcontrib>Salomon, David S.</creatorcontrib><title>Transformation of mouse mammary epithelial cells with the Ha- ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-
ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21
ras
expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for
ras because
neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cell Line, Transformed</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Cell Transformation, Neoplastic - metabolism</subject><subject>Cells, Cultured</subject><subject>CM, conditioned medium</subject><subject>DMEM, Dulbecco's modified Eagle's medium</subject><subject>EGF, epidermal growth factor</subject><subject>Epithelial Cells</subject><subject>Epithelium - metabolism</subject><subject>FBS, fetal bovine serum</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Mammary Glands, Animal - cytology</subject><subject>Mammary Glands, Animal - metabolism</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Oncogene</subject><subject>Oncogene Protein p21(ras)</subject><subject>Oncogene Proteins, Viral - genetics</subject><subject>Oncogene, neu</subject><subject>Oncogene, ras</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogenes</subject><subject>Receptor, ErbB-2</subject><subject>RIA, radioimmunoassay</subject><subject>RNA, Messenger - analysis</subject><subject>RRA, radioreceptor assay</subject><subject>TGFα, transforming growth factor-α</subject><subject>Transformation</subject><subject>Transforming growth factor-α</subject><subject>Transforming Growth Factors - biosynthesis</subject><subject>Viral Proteins - genetics</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNqNUcFu1DAQtRCoLIU_AMkXED0E7Dhx7AsSVF2KVIlLOVu2M1mMEnuxHVb9LE78Bd9UZ3dpb8DJmnlv3ozfQ-g5JW8oofwtIbSp2k6y10KeCdJ1smIP0IqKjlWs4eIhWt1RHqMnKX0jpRZUnqCTuuVCNnKFfl1H7dMQ4qSzCx6HAU9hToAnPU063mDYuvwVRqdHbGEcE96VGpcWvtQVjjphM2fsQ74HPMw4eBs24AFHSPOYE3Yea7zv9CHpDVQ9bMH34HOBbARddhZO_nOO8xu8iWFXNAdtc4jV7594G0M_2-XQp-jRoMcEz47vKfqyvrg-v6yuPn_8dP7-qrJtXXwwgrWUSgOkawwlBlg9dNAPxnDKqWHQApWdEb3gmnZiYMJIMfSGM6Nr2Rh2il4ddMvq7zOkrCaXFiO0h-KT6iQlnNPun0TaMkqkqAuxORBtDClFGNQ2usVqRYlaglVLampJTQmp9sEqVsZeHPVnM0F_N3RMsuAvj7hOVo9D8dG6dK8taylaTgtvfeDt3Ag3_7VbrS8-1Auw9IXcd5eD3h2EoNj_w0FUyTrwFnoXwWbVB_f3H90C5bXXdA</recordid><startdate>19890703</startdate><enddate>19890703</enddate><creator>Ciardiello, Fortunato</creator><creator>Hynes, Nancy</creator><creator>Kim, Nancy</creator><creator>Valverius, Eva M.</creator><creator>Lippman, Marc E.</creator><creator>Salomon, David S.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19890703</creationdate><title>Transformation of mouse mammary epithelial cells with the Ha- ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production</title><author>Ciardiello, Fortunato ; Hynes, Nancy ; Kim, Nancy ; Valverius, Eva M. ; Lippman, Marc E. ; Salomon, David S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5273-b835119be074b10be32f7edfbb6161b3e5e197b8d86a178f38b98fdb63ba294b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cell Line, Transformed</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Cell Transformation, Neoplastic - metabolism</topic><topic>Cells, Cultured</topic><topic>CM, conditioned medium</topic><topic>DMEM, Dulbecco's modified Eagle's medium</topic><topic>EGF, epidermal growth factor</topic><topic>Epithelial Cells</topic><topic>Epithelium - metabolism</topic><topic>FBS, fetal bovine serum</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Mammary Glands, Animal - cytology</topic><topic>Mammary Glands, Animal - metabolism</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Oncogene</topic><topic>Oncogene Protein p21(ras)</topic><topic>Oncogene Proteins, Viral - genetics</topic><topic>Oncogene, neu</topic><topic>Oncogene, ras</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogenes</topic><topic>Receptor, ErbB-2</topic><topic>RIA, radioimmunoassay</topic><topic>RNA, Messenger - analysis</topic><topic>RRA, radioreceptor assay</topic><topic>TGFα, transforming growth factor-α</topic><topic>Transformation</topic><topic>Transforming growth factor-α</topic><topic>Transforming Growth Factors - biosynthesis</topic><topic>Viral Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ciardiello, Fortunato</creatorcontrib><creatorcontrib>Hynes, Nancy</creatorcontrib><creatorcontrib>Kim, Nancy</creatorcontrib><creatorcontrib>Valverius, Eva M.</creatorcontrib><creatorcontrib>Lippman, Marc E.</creatorcontrib><creatorcontrib>Salomon, David S.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ciardiello, Fortunato</au><au>Hynes, Nancy</au><au>Kim, Nancy</au><au>Valverius, Eva M.</au><au>Lippman, Marc E.</au><au>Salomon, David S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transformation of mouse mammary epithelial cells with the Ha- ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1989-07-03</date><risdate>1989</risdate><volume>250</volume><issue>2</issue><spage>474</spage><epage>478</epage><pages>474-478</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-
ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21
ras
expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for
ras because
neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2568949</pmid><doi>10.1016/0014-5793(89)80779-3</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1989-07, Vol.250 (2), p.474-478 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | ScienceDirect |
| subjects | Animals Biological and medical sciences Blotting, Northern Cell Line, Transformed Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Cell Transformation, Neoplastic - metabolism Cells, Cultured CM, conditioned medium DMEM, Dulbecco's modified Eagle's medium EGF, epidermal growth factor Epithelial Cells Epithelium - metabolism FBS, fetal bovine serum Fundamental and applied biological sciences. Psychology Gene Expression Regulation Mammary Glands, Animal - cytology Mammary Glands, Animal - metabolism Mice Molecular and cellular biology Oncogene Oncogene Protein p21(ras) Oncogene Proteins, Viral - genetics Oncogene, neu Oncogene, ras Proto-Oncogene Proteins - genetics Proto-Oncogenes Receptor, ErbB-2 RIA, radioimmunoassay RNA, Messenger - analysis RRA, radioreceptor assay TGFα, transforming growth factor-α Transformation Transforming growth factor-α Transforming Growth Factors - biosynthesis Viral Proteins - genetics |
| title | Transformation of mouse mammary epithelial cells with the Ha- ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production |
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