Impaired cellular cholesterol efflux by oxysterol-enriched high density lipoproteins

One of the proposed antiatherogenicity role of high-density lipoproteins (HDL) is believed to stimulate removal of cholesterol from the peripheral cells back to the liver for excretion. We have investigated the effects of oxidation-related modifications of HDL on their ability to stimulate cholester...

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Bibliographic Details
Published in:Free radical biology & medicine 1997, Vol.23 (4), p.541-547
Main Authors: Gesquière, L, Loreau, N, Blache, D
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cholesterol - metabolism
Chromatography, Gas
Copper - pharmacology
Humans
Hydroxycholesterols - pharmacology
Ketocholesterols - pharmacology
Lipoproteins, HDL - metabolism
Macrophages - metabolism
Malondialdehyde - pharmacology
Mice
Oxidation-Reduction
Thiobarbituric Acid Reactive Substances - metabolism
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Summary:One of the proposed antiatherogenicity role of high-density lipoproteins (HDL) is believed to stimulate removal of cholesterol from the peripheral cells back to the liver for excretion. We have investigated the effects of oxidation-related modifications of HDL on their ability to stimulate cholesterol efflux from cultured cells. Human HDL (HDL3, 1.13 < d < 1.21 g/ml) have been modified either by malondialdehyde or by copper-mediated oxidation (Ox-HDL3). Compared with native HDL3, the modified HDL3 resulted in a significantly reduced efflux of labeled cholesterol from preloaded macrophages (P388D1 cell line). Analysis of lipid composition of Ox-HDL3 by gas chromatography revealed the presence of oxysterols (OS). Enrichment of native HDL3 with oxysterols resulted in a reduced capacity to stimulate cholesterol efflux. The reduced ability of OS-enriched HDL3 to elicit cholesterol efflux may contribute to cellular cholesterol accumulation and subsequently to atherosclerosis.
ISSN:0891-5849
DOI:10.1016/S0891-5849(97)00114-7