Detection of Clonal T Cell Populations with Closely Related T Cell Receptor Junctional Sequences in Persons at High Risk for Human Immunodeficiency Virus (HIV) Infection and in Patients Acutely Infected with HIV

The T cell repertoires were characterized for CD4+ and CD4− lymphocytes derived from 2 patients with acute human immunodeficiency virus (HIV) infection and from 2S HIV-seronegative persons at high risk for acquiring HIV. Oligoclonal expansions of CD4− cells were detected in the HIV-infected patients...

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Bibliographic Details
Published in:The Journal of infectious diseases 1997-02, Vol.175 (2), p.272-282
Main Authors: Bettinardi, Alessandra, Imberti, Luisa, Sottini, Alessandra, Quiròs-Roldan, Eugenia, Puoti, Massimo, Castelli, Francesco, Cadeo, Gian Pietro, Gorla, Roberto, Primi, Daniele
Format: Article
Language:English
Subjects:
AIDS/HIV
Amino acids
Biological and medical sciences
CD4 Lymphocyte Count
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Cell Division - immunology
Cells, Cultured
Clone Cells - immunology
Complementary DNA
Female
Flow Cytometry
HIV
HIV 1
HIV infections
HIV Infections - genetics
HIV Infections - immunology
HIV Seronegativity
human immunodeficiency virus
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Infections
Lymphocytes
Major Articles
Male
Medical sciences
Polymerase Chain Reaction
Receptors
Receptors, Antigen, T-Cell, alpha-beta - genetics
Receptors, Antigen, T-Cell, alpha-beta - immunology
Sequence Analysis, DNA
Sexual Partners
T cell antigen receptors
T lymphocytes
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Description
Summary:The T cell repertoires were characterized for CD4+ and CD4− lymphocytes derived from 2 patients with acute human immunodeficiency virus (HIV) infection and from 2S HIV-seronegative persons at high risk for acquiring HIV. Oligoclonal expansions of CD4− cells were detected in the HIV-infected patients and in 2 of 3 uninfected high-risk subjects with a reduced number of CD4+ lymphocytes. Furthermore, nucleotide sequencing revealed that some of the T cell receptor (TCR) beta variable segments (TCRBV), which were highly selected in the high-risk subjects, shared closely related junctional sequences, with the TCRBV predominantly expanded in the HIV-infected patients. Since the likelihood that these similarities occurred by chance is extremely low, these data provide direct molecular evidence in support of several cellular and serologic studies suggesting that some persons remain uninfected despite exposure to HIV.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/175.2.272