Optimized methods for capillary electrophoresis of tetracyclines

Optimized methods for the analysis of some tetracyclines by capillary electrophoresis are described. Different buffer systems were employed for the separation of tetracycline, oxytetracycline and demeclocycline from their respective major impurities, including the 2-acetyl-2-decarboxamido derivative...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1997-05, Vol.15 (8), p.1063-1069
Main Authors: Li, Y.M., Van Schepdael, A., Roets, E., Hoogmartens, J.
Format: Article
Language:English
Subjects:
2-Acetyl-2-decarboxamido derivatives
Buffers
Capillary electrophoresis
Chromatography, Liquid
Cyclodextrins
Demeclocycline
Demeclocycline - analogs & derivatives
Demeclocycline - analysis
Drug Contamination
Electrophoresis, Capillary
Hydrogen-Ion Concentration
Octoxynol
Oxytetracycline
Oxytetracycline - analysis
Tetracycline
Tetracycline - analysis
Tetracyclines - analysis
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Summary:Optimized methods for the analysis of some tetracyclines by capillary electrophoresis are described. Different buffer systems were employed for the separation of tetracycline, oxytetracycline and demeclocycline from their respective major impurities, including the 2-acetyl-2-decarboxamido derivatives. The influence of buffer pH and buffer concentration was systematically investigated. Non-ionic surfactant Triton X-100 and methyl- β-cyclodextrin were used to obtain improved selectivity in the case of oxytetracycline and demeclocycline. The results are compared with those of previously established liquid chromatography methods. Good correlations were obtained.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(96)01995-4