Human papillomavirus is more prevalent in first trimester spontaneously aborted products of conception compared to elective specimens

In this study the possible role of human papillomaviruses (HPV) in spontaneous abortions is addressed by assaying for HPV DNA in first trimester spontaneous and electively aborted products of conception materials enriched for chorionic villi. The presence of HPVs was measured by polymerase chain rea...

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Bibliographic Details
Published in:Virus genes 1997, Vol.14 (1), p.13-17
Main Authors: Hermonat, P L, Han, L, Wendel, P J, Quirk, J G, Stern, S, Lowery, C L, Rechtin, T M
Format: Article
Language:English
Subjects:
Abortion, Induced
Abortion, Spontaneous - etiology
Abortion, Spontaneous - virology
DNA, Viral - genetics
DNA, Viral - isolation & purification
Female
Globins - genetics
Humans
Papillomaviridae - genetics
Papillomaviridae - isolation & purification
Papillomaviridae - pathogenicity
Papillomavirus Infections - complications
Polymerase Chain Reaction
Pregnancy
Pregnancy Complications, Infectious - virology
Pregnancy Trimester, First
Tumor Virus Infections - complications
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Summary:In this study the possible role of human papillomaviruses (HPV) in spontaneous abortions is addressed by assaying for HPV DNA in first trimester spontaneous and electively aborted products of conception materials enriched for chorionic villi. The presence of HPVs was measured by polymerase chain reaction (PCR) amplification and DNA dot blot hybridization using an internal probe. The "broad spectrum" HPV primers were directed to amplify E6/E7 junction sequences, while the probe was of an HPV-16 sequence with significant homology to HPV-6/11. The quantity and quality of isolated DNA was also analyzed and compared by observing the PCR amplification of a cellular sequence from the human beta-globin gene. Fifteen of the 25 spontaneous samples (60%) were found to be positive for HPV E6/E7 sequences. In comparison, only 3 of the 15 elective samples (20%) were positive. This is the first study of HPV in fetal materials to incorporate material from elective abortions as a control group. Although confounding contamination from the cervix and vagina can't be ruled out, these data are significant and strongly suggest that HPVs are elevated in spontaneously aborted products of conception. Furthermore, these results suggest the possibility that HPVs may be etiologic agents of at least some spontaneous abortions.
ISSN:0920-8569
DOI:10.1023/A:1007975005433