Lutropin-Choriogonadotropin Receptor: An Unusual Member of the G Protein--Coupled Receptor Family

A complementary DNA (cDNA) for the rat luteal lutropin-choriogonadotropin receptor (LH-CG-R) was isolated with the use of a DNA probe generated in a polymerase chain reaction with oligonucleotide primers based on peptide sequences of purified receptor protein. As would be predicted from the cDNA seq...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1989-08, Vol.245 (4917), p.494-499
Main Authors: McFarland, K. C., Sprengel, Rolf, Phillips, Heidi S., Köhler, Martin, Rosemblit, Nora, Nikolics, Karoly, Segaloff, Deborah L., Seeburg, Peter H.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Cloning, Molecular
Complementary DNA
DNA
DNA - genetics
DNA - isolation & purification
DNA Probes
Female
G proteins
Generally accepted auditing standards
Genetics
Glycoproteins - genetics
GTP-Binding Proteins - physiology
Hormone receptors
Hormones
Interstitial cells
Luteinizing hormone
Messenger RNA
Molecular Sequence Data
Nucleic Acid Hybridization
Ovaries
Ovary - analysis
Proteins
Rats
Receptors
Receptors, LH - genetics
RNA
RNA, Messenger - analysis
RNA, Messenger - genetics
Sequence Homology, Nucleic Acid
Tissue Distribution
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Summary:A complementary DNA (cDNA) for the rat luteal lutropin-choriogonadotropin receptor (LH-CG-R) was isolated with the use of a DNA probe generated in a polymerase chain reaction with oligonucleotide primers based on peptide sequences of purified receptor protein. As would be predicted from the cDNA sequence, the LH-CG-R consists of a 26-residue signal peptide, a 341-residue extracellular domain displaying an internal repeat structure characteristic of members of the leucine-rich glycoprotein (LRG) family, and a 333-residue region containing seven transmembrane segments. This membrane-spanning region displays sequence similarity with all members of the G protein--coupled receptor family. Hence, the LH-CG-R gene may have evolved by recombination of LRG and G protein--coupled receptor genes. Cells engineered to express LH-CG-R cDNA bind human choriogonadotropin with high affinity and show an increase in cyclic adenosine monophosphate when exposed to hormone. As revealed by RNA blot analysis and in situ hybridization, the 4.4-kilobase cognate messenger RNA is prominently localized in the rat ovary.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2502842