SPECIFIC DETECTION OF κ LIGHT CHAIN IN URIC ACID STONES

Proteins were extracted from uric acid stones with 6M guanidine chloride (pH 8.5), which were successively developed by 12% polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). Amino acid sequence analysis of each band on SDS-PAGE revealed that major components in...

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Bibliographic Details
Published in:Life sciences (1973) 1997, Vol.61 (3), p.249-253
Main Authors: Hashimoto, Yoshihiro, Kohri, Kenjiro, Hayashi, Yutaro, Moriyama, Akihiko, Iguchi, Masanori, Asai, Kiyofumi, Kato, Taiji
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Guanidine
Guanidines
Hemoglobin A - chemistry
Humans
Immunoglobulin Heavy Chains - chemistry
Immunoglobulin kappa-Chains - analysis
Immunoglobulin kappa-Chains - chemistry
immunoglobulins
Molecular Sequence Data
Sequence Homology
Uric Acid - analysis
uric acid stone
Urinary Calculi - chemistry
Urinary Calculi - immunology
κ light chain
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Summary:Proteins were extracted from uric acid stones with 6M guanidine chloride (pH 8.5), which were successively developed by 12% polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). Amino acid sequence analysis of each band on SDS-PAGE revealed that major components in uric acid stones were immunoglobulin α heavy and κ light chains. Although immunoglobulin heavy chain (γ and μ, as well as α) and a κ light chain were clearly detected in uric acid stones by Western blotting using their specific antibodies, no λ chains whatsoever could be detected. The results suggest that immunoglobulins selectively containing κ light chain might have specific functions in uric acid stone formation as stone matrices.
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(97)00380-9