The yeast spindle pole body is assembled around a central crystal of Spc42p

The spindle pole body (SPB) is the microtubule organizing center (MTOC) in the yeast Saccharomyces that plays a pivotal role in such diverse processes as mitosis, budding, and mating. We have used cryoelectron microscopy and image processing to study the structure of isolated diploid SPBs. We show t...

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Bibliographic Details
Published in:Cell 1997-06, Vol.89 (7), p.1077-1086
Main Authors: Bullitt, E, Rout, M P, Kilmartin, J V, Akey, C W
Format: Article
Language:English
Subjects:
Centrosome - chemistry
Centrosome - ultrastructure
Crystallography
Diploidy
Fungal Proteins - analysis
Fungal Proteins - chemistry
Gene Expression Regulation, Fungal - physiology
Microscopy, Electron
Saccharomyces cerevisiae
Saccharomyces cerevisiae - chemistry
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - ultrastructure
Spindle Apparatus - chemistry
Spindle Apparatus - ultrastructure
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Summary:The spindle pole body (SPB) is the microtubule organizing center (MTOC) in the yeast Saccharomyces that plays a pivotal role in such diverse processes as mitosis, budding, and mating. We have used cryoelectron microscopy and image processing to study the structure of isolated diploid SPBs. We show that SPBs are present in two lateral-size classes, sharing a similar vertical architecture comprised of six major layers. Tomographic reconstructions of heparin-stripped SPBs reveal a central hexagonally packed layer. Overexpression of Spc42p results in the growth of a similar layer, forming a crystal that encircles the SPB. Hence, the SPB is an MTOC that utilizes crystallographic packing of subunits in its construction.
ISSN:0092-8674
DOI:10.1016/S0092-8674(00)80295-0