Bovine cytokine expression during different phases of bovine leukemia virus infection

The potential role of aberrant cytokine production in the pathogenesis of bovine leukemia virus (BLV) was studied by analyzing cytokine mRNA expression in pokeweed-stimulated PBMLs of cows in different phases of disease progression. To analyze the mRNA, a semi-quantitative RT-PCR assay was developed...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 1997-05, Vol.56 (1), p.39-51
Main Authors: Keefe, Robert G., Choi, Yeon, Ferrick, David A., Stott, Jeffrey L.
Format: Article
Language:English
Subjects:
AIDS/HIV
Animals
ARN MENSAJERO
ARN MESSAGER
BOVIN
BOVINE LEUKAEMIA VIRUS
Bovine leukemia virus
CATTLE
Cattle Diseases - immunology
Cells, Cultured
CITOQUINAS
CYTOKINE
CYTOKINES
Cytokines - biosynthesis
Cytokines - genetics
Enzootic Bovine Leukosis - genetics
Enzootic Bovine Leukosis - metabolism
Enzootic Bovine Leukosis - virology
Female
GANADO BOVINO
IMMUNE RESPONSE
Leukemia Virus, Bovine
Lymphocyte Activation
MESSENGER RNA
PATHOGENICITY
PATOGENICIDAD
PCR
Pokeweed Mitogens
Polymerase chain reaction
Polymerase Chain Reaction - veterinary
POUVOIR PATHOGENE
REPONSE IMMUNITAIRE
RESPUESTA INMUNOLOGICA
RNA, Messenger - biosynthesis
RT-PCR
Transcription, Genetic
Tumor Cells, Cultured
VIRUS LEUCEMIA BOVINA
VIRUS LEUCEMIE BOVINE
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Summary:The potential role of aberrant cytokine production in the pathogenesis of bovine leukemia virus (BLV) was studied by analyzing cytokine mRNA expression in pokeweed-stimulated PBMLs of cows in different phases of disease progression. To analyze the mRNA, a semi-quantitative RT-PCR assay was developed. The RT-PCR assay was developed for detection of IL-2, -4, -6, -10, -12, IFN-γ and actin using cDNA derived from phorbol-stimulated peripheral blood mononuclear leukocytes. Using a PCR specific for BLV tax, agar gel immunodiffusion and white blood cell counts, BLV-negative, BLV-positive aleukemic (AL), and BLV-positive persistently lymphocytotic (PL) cattle were identified. Peripheral blood lymphocytes cultured in vitro for 24 h in pokeweed mitogen were analyzed for cytokine production using the RT-PCR assay. Consistently elevated levels of IL-2 and IL-12 in AL and PL cattle in pokeweed mitogen-stimulated cells was detected, while IFN-γ was elevated in the AL but not the PL cattle.
ISSN:0165-2427
1873-2534
DOI:10.1016/S0165-2427(96)05727-3