coupling of cyclic GMP and photopolarization of Pelvetia zygotes

Unidirectional blue light directs the rhizoid-thallus axis in the apolar zygotes of Fucus and Pelvetia. Here, it is shown that blue light (but not red light, increased cyclic GMP levels of Pelvetia zygotes by about a factor of 2. When the increase in cyclic GMP was blocked by a guanylyl cyclase inhi...

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Bibliographic Details
Published in:Developmental biology 1997-07, Vol.187 (1), p.125-130
Main Authors: Robinson, K.R, Miller, B.J
Format: Article
Language:English
Subjects:
1-Methyl-3-isobutylxanthine - pharmacology
Adenine - analogs & derivatives
Adenine - pharmacology
Adenylyl Cyclase Inhibitors
Aminoquinolines - pharmacology
Cell Division
Cell Polarity - drug effects
cyclic GMP
Cyclic GMP - metabolism
Enzyme Inhibitors - pharmacology
Guanylate Cyclase - antagonists & inhibitors
Light
Phaeophyceae - cytology
Phaeophyceae - drug effects
Phaeophyceae - physiology
Phosphoric Diester Hydrolases - metabolism
Zygote - physiology
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Summary:Unidirectional blue light directs the rhizoid-thallus axis in the apolar zygotes of Fucus and Pelvetia. Here, it is shown that blue light (but not red light, increased cyclic GMP levels of Pelvetia zygotes by about a factor of 2. When the increase in cyclic GMP was blocked by a guanylyl cyclase inhibitor, photopolarization was also blocked. Bathing the cells in a permeant cyclic GMP analog, which should tend to collapse intracellular cyclic GMP gradients, reduced the degree of photopolarization. Growing the cells in the dark in a gradient of the analog caused the rhizoids to tend to form on the low concentration side. It appears that the stimulation of the blue light photoreceptors on the side nearer the light activates guanylyl cyclase and results in a transcytoplasmic cyclic GMP gradient that is necessary for polarization.
ISSN:0012-1606
1095-564X
DOI:10.1006/dbio.1997.8600