Isolation and characterization of Vibrio carchariae, a causative agent of gastroenteritis in the groupers, Epinephelus coioides

An outbreak of serious mortality among the cultured groupers Epinephelus coioides, characterized by a swollen intestine containing yellow fluid, occurred in the summer of 1993 in Taiwan. A motile strain EmI82KL was isolated from the intestinal yellow fluid of the moribund groupers with tryptic soy a...

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Bibliographic Details
Published in:Current microbiology 1997-08, Vol.35 (2), p.109-115
Main Authors: Yii, K.C. (National Taiwan Ocean University, Keelung, Taiwan, ROC.), Yang, T.I, Lee, K.K
Format: Article
Language:English
Subjects:
Animal bacterial diseases
Animal productions
Animals
Anti-Bacterial Agents - pharmacology
Aquaculture
Bacterial diseases
Bacteriological Techniques
Biological and medical sciences
Biotechnology
Body weight
EPINEPHELUS
Epinephelus coioides
Fish
Fish Diseases - metabolism
Fishes - microbiology
Fundamental and applied biological sciences. Psychology
GASTROENTERITE
GASTROENTERITIS
Gastroenteritis - microbiology
Gastroenteritis - veterinary
Hemagglutination
Hemolysin Proteins - metabolism
Infectious diseases
Marine
Medical sciences
OUTBREAKS
Rabbits
Sheep
Sodium chloride
TAIWAN
Terrestrial animal productions
Vertebrates
Vibrio - classification
Vibrio - drug effects
Vibrio - isolation & purification
Vibrio carchariae
VIBRIO CHOLERAE
Vibrio Infections - veterinary
VIBRIOSIS
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Summary:An outbreak of serious mortality among the cultured groupers Epinephelus coioides, characterized by a swollen intestine containing yellow fluid, occurred in the summer of 1993 in Taiwan. A motile strain EmI82KL was isolated from the intestinal yellow fluid of the moribund groupers with tryptic soy agar supplemented with 2% NaCl and/or thiosulfate citrate bile salt sucrose agar. This strain was characterized and identified as Vibrio carchariae and was susceptible to chloramphenicol, doxycycline-HCl, nalidixic acid, oxolinic acid, oxytetracycline, and sulfonamide while resistant to ampicillin and penicillin G. In addition, the strain was neither auto-agglutinating nor hemagglutinating, but it was hemolytic against erythrocytes from sheep, rabbit, tilapia, and grouper. The bacteria could be reisolated from kidney, liver, and the transparent yellow fluid of swollen intestine of moribund groupers after bacterial challenge and re-identified as the same species. The LD50 value was 2.53 x 10(7) colony forming units/g grouper body weight
ISSN:0343-8651
1432-0991
DOI:10.1007/s002849900221