AtMRP1 gene of Arabidopsis encodes a glutathione S-conjugate pump: isolation and functional definition of a plant ATP-binding cassette transporter gene

• Published in: Proceedings of the National Academy of Sciences - PNAS 1997-07, Vol.94 (15), p.8243-8248
• Main Authors: Lu, Y.P. (University of Pennsylvania, Philadelphia, PA.), Li, Z.S, Rea, P.A
• Format: Article
• Language: English
• Subjects: 2,4-DINITROFENOL; 2,4-DINITROPHENOL; ACTIVE TRANSPORT; ADENOSINETRIPHOSPHATASE; ADN; Amino Acid Sequence; AMINO ACID SEQUENCES; Amino acids; Arabidopsis - genetics; ARABIDOPSIS THALIANA; ARN MENSAJERO; ARN MESSAGER; ATP; ATP binding cassette transporters; ATP-Binding Cassette Transporters - chemistry; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette Transporters - metabolism; ATPASA; ATPASE; BINDING PROTEINS; Biological Sciences; Biological Transport; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - metabolism; Catalysis; CELL MEMBRANES; CHEMICAL COMPOSITION; Chromosome Mapping; Cloning, Molecular; COMPLEMENTARY DNA; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; DINITROPHENOL-GLUTATHIONE CONJUGATE; DNA; EXPRESION GENICA; EXPRESSION DES GENES; GENBANK/AF008124; GENBANK/AF008125; GENE; GENE EXPRESSION; GENES; Genes, Plant; Genomics; GLUTATHION; GLUTATHIONE; GLUTATION; MEMBRANAS CELULARES; MEMBRANE CELLULAIRE; Membrane Transport Proteins; MESSENGER RNA; MOLECULAR SEQUENCE DATA; NUCLEOTIDE SEQUENCE; P branes; Plants; Polymerase chain reaction; PROTEINAS AGLUTINANTES; PROTEINE DE LIAISON; Pumps; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; STRUCTURAL GENES; TONOPLAST; Vacuoles - metabolism; Vanadates; Yeasts
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.94.15.8243

Because plants produce cytotoxic compounds to which they, themselves, are susceptible and are exposed to exogenous toxins (microbial products, allelochemicals, and agrochemicals), cell survival is contingent on mechanisms for detoxifying these agents. One detoxification mechanism is the glutathione S-transferase-catalyzed glutathionation of the toxin, or an activated derivative, and transport of the conjugate out of the cytosol. We show here that a transporter responsible for the removal of glutathione S-conjugates from the cytosol, a specific Mg2+-ATPase, is encoded by the AtMRP1 gene of Arabidopsis thaliana. The sequence of AtMRP1 and the transport capabilities of membranes prepared from yeast cells transformed with plasmid-borne AtMRP1 demonstrate that this gene encodes an ATP-binding cassette transporter competent in the transport of glutathione S-conjugates of xenobiotics and endogenous substances, including herbicides and anthocyanins