Ribotyping and DNA macrorestriction analysis of isolates of Burkholderia pseudomallei from cases of melioidosis in Malaysia

Forty-nine isolates of Burkholderia pseudomallei from sporadic cases of melioidosis in Malaysia over the past 18 years were examined by BamHI ribotyping and pulsed-field gel electrophoresis (PFGE) of XbaI digests of total deoxyribonucleic acid (DNA). Twenty-four patients had septicaemic melioidosis...

Full description

Saved in:
Bibliographic Details
Published in:Transactions of the Royal Society of Tropical Medicine and Hygiene 1997-05, Vol.91 (3), p.358-360
Main Authors: Vadivelu, Jamuna, Puthucheary, S.D., Mifsud, A., Drasar, B.S., Dance, D.A.B., Pitt, T.L.
Format: Article
Language:English
Subjects:
Adult
Aged
Bacterial diseases
Biological and medical sciences
Burkholderia pseudomallei
Burkholderia pseudomallei - genetics
Deoxyribonuclease BamHI
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Electrophoresis, Gel, Pulsed-Field
Female
genotypes
Human bacterial diseases
Humans
Infectious diseases
Malaysia
Male
Medical sciences
Melioidosis
Melioidosis - microbiology
Middle Aged
Restriction Mapping
ribotypes
Tropical bacterial diseases
Tropical medicine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Forty-nine isolates of Burkholderia pseudomallei from sporadic cases of melioidosis in Malaysia over the past 18 years were examined by BamHI ribotyping and pulsed-field gel electrophoresis (PFGE) of XbaI digests of total deoxyribonucleic acid (DNA). Twenty-four patients had septicaemic melioidosis with a mortality of 70%; mortality in the non-septicaemic disease was 16%. Five ribotype patterns were identified, 2 of which accounted for 90% of all isolates. PFGE revealed a number of different strains within these ribotypes, but some pairs of isolates from unrelated cases gave closely similar DNA profiles. These results are in agreement with Australian studies which showed a high prevalence of a few ribotypes of B. pseudomallei which are further divisible by genotyping, in areas where melioidosis is endemic.
ISSN:0035-9203
1878-3503
DOI:10.1016/S0035-9203(97)90107-3