Interferon-Gamma Production by the Human Preimplantation Embryo

PROBLEM: This study demonstrated that the human embryo produces interferon‐gamma (IFNγ). It is important to know whether IFNγ can be produced before implantation. Therefore the aim of this study was to evaluate the profile of IFNγ production between days 2 and 5 after in vitro fertilization. METHOD:...

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Published in:American journal of reproductive immunology (1989) 1997-06, Vol.37 (6), p.435-437
Main Authors: ÖZörnek, M. H., Bielfeld, P., Krüssel, J. S., Copisti, S., Jeyendran, R. S., Koldovsky, U.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blastocyst - metabolism
Enzyme-Linked Immunosorbent Assay
Female
Fertilization in Vitro
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental
HLA-G
Hormone metabolism and regulation
human embryo
Humans
interferon gamma
Interferon-gamma - biosynthesis
Interferon-gamma - genetics
Male
Pregnancy. Parturition. Lactation
Vertebrates: reproduction
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cited_by cdi_FETCH-LOGICAL-c4696-c9002e487630b86b42d76c060e36ab755ba4abf7ff29c4afb9c37255f2313f943
cites cdi_FETCH-LOGICAL-c4696-c9002e487630b86b42d76c060e36ab755ba4abf7ff29c4afb9c37255f2313f943
container_end_page 437
container_issue 6
container_start_page 435
container_title American journal of reproductive immunology (1989)
container_volume 37
creator ÖZörnek, M. H.
Bielfeld, P.
Krüssel, J. S.
Copisti, S.
Jeyendran, R. S.
Koldovsky, U.
description PROBLEM: This study demonstrated that the human embryo produces interferon‐gamma (IFNγ). It is important to know whether IFNγ can be produced before implantation. Therefore the aim of this study was to evaluate the profile of IFNγ production between days 2 and 5 after in vitro fertilization. METHOD: Twenty embryos were cultured from day 2 to 5 after fertilization. The embryo stages were checked each day and the media refreshed. IFNγ levels were estimated by an enzyme‐linked immunosorbent assay. RESULTS: All embryos produced measurable IFNγ at least for 1 day. Yields of IFNγ were: 0.46 ± 0.45 (n = 4) on day 2, 0.69 ± 0.52 (n = 19) on day 3, 0.73 ± 0.52 (n = 15) on day 4, 0.55 ± 0.32 (n = 11) IU/ml on day 5, respectively. There was no significant difference in the IFNγ production between in vitro culture days or between the developmental stages of embryos. CONCLUSION: IFNγ is produced by all the embryos and seems to peak between days 3 and 4, which is just before implantation.
doi_str_mv 10.1111/j.1600-0897.1997.tb00256.x
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H. ; Bielfeld, P. ; Krüssel, J. S. ; Copisti, S. ; Jeyendran, R. S. ; Koldovsky, U.</creator><creatorcontrib>ÖZörnek, M. H. ; Bielfeld, P. ; Krüssel, J. S. ; Copisti, S. ; Jeyendran, R. S. ; Koldovsky, U.</creatorcontrib><description>PROBLEM: This study demonstrated that the human embryo produces interferon‐gamma (IFNγ). It is important to know whether IFNγ can be produced before implantation. Therefore the aim of this study was to evaluate the profile of IFNγ production between days 2 and 5 after in vitro fertilization. METHOD: Twenty embryos were cultured from day 2 to 5 after fertilization. The embryo stages were checked each day and the media refreshed. IFNγ levels were estimated by an enzyme‐linked immunosorbent assay. RESULTS: All embryos produced measurable IFNγ at least for 1 day. Yields of IFNγ were: 0.46 ± 0.45 (n = 4) on day 2, 0.69 ± 0.52 (n = 19) on day 3, 0.73 ± 0.52 (n = 15) on day 4, 0.55 ± 0.32 (n = 11) IU/ml on day 5, respectively. There was no significant difference in the IFNγ production between in vitro culture days or between the developmental stages of embryos. CONCLUSION: IFNγ is produced by all the embryos and seems to peak between days 3 and 4, which is just before implantation.</description><identifier>ISSN: 1046-7408</identifier><identifier>ISSN: 8755-8920</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/j.1600-0897.1997.tb00256.x</identifier><identifier>PMID: 9228298</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Blastocyst - metabolism ; Enzyme-Linked Immunosorbent Assay ; Female ; Fertilization in Vitro ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; HLA-G ; Hormone metabolism and regulation ; human embryo ; Humans ; interferon gamma ; Interferon-gamma - biosynthesis ; Interferon-gamma - genetics ; Male ; Pregnancy. 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H.</creatorcontrib><creatorcontrib>Bielfeld, P.</creatorcontrib><creatorcontrib>Krüssel, J. S.</creatorcontrib><creatorcontrib>Copisti, S.</creatorcontrib><creatorcontrib>Jeyendran, R. S.</creatorcontrib><creatorcontrib>Koldovsky, U.</creatorcontrib><title>Interferon-Gamma Production by the Human Preimplantation Embryo</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>PROBLEM: This study demonstrated that the human embryo produces interferon‐gamma (IFNγ). It is important to know whether IFNγ can be produced before implantation. Therefore the aim of this study was to evaluate the profile of IFNγ production between days 2 and 5 after in vitro fertilization. METHOD: Twenty embryos were cultured from day 2 to 5 after fertilization. The embryo stages were checked each day and the media refreshed. IFNγ levels were estimated by an enzyme‐linked immunosorbent assay. RESULTS: All embryos produced measurable IFNγ at least for 1 day. Yields of IFNγ were: 0.46 ± 0.45 (n = 4) on day 2, 0.69 ± 0.52 (n = 19) on day 3, 0.73 ± 0.52 (n = 15) on day 4, 0.55 ± 0.32 (n = 11) IU/ml on day 5, respectively. There was no significant difference in the IFNγ production between in vitro culture days or between the developmental stages of embryos. CONCLUSION: IFNγ is produced by all the embryos and seems to peak between days 3 and 4, which is just before implantation.</description><subject>Biological and medical sciences</subject><subject>Blastocyst - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>HLA-G</subject><subject>Hormone metabolism and regulation</subject><subject>human embryo</subject><subject>Humans</subject><subject>interferon gamma</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interferon-gamma - genetics</subject><subject>Male</subject><subject>Pregnancy. Parturition. 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RESULTS: All embryos produced measurable IFNγ at least for 1 day. Yields of IFNγ were: 0.46 ± 0.45 (n = 4) on day 2, 0.69 ± 0.52 (n = 19) on day 3, 0.73 ± 0.52 (n = 15) on day 4, 0.55 ± 0.32 (n = 11) IU/ml on day 5, respectively. There was no significant difference in the IFNγ production between in vitro culture days or between the developmental stages of embryos. CONCLUSION: IFNγ is produced by all the embryos and seems to peak between days 3 and 4, which is just before implantation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9228298</pmid><doi>10.1111/j.1600-0897.1997.tb00256.x</doi><tpages>3</tpages></addata></record>
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ispartof American journal of reproductive immunology (1989), 1997-06, Vol.37 (6), p.435-437
issn 1046-7408
8755-8920
1600-0897
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source Wiley-Blackwell Read & Publish Collection
subjects Biological and medical sciences
Blastocyst - metabolism
Enzyme-Linked Immunosorbent Assay
Female
Fertilization in Vitro
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental
HLA-G
Hormone metabolism and regulation
human embryo
Humans
interferon gamma
Interferon-gamma - biosynthesis
Interferon-gamma - genetics
Male
Pregnancy. Parturition. Lactation
Vertebrates: reproduction
title Interferon-Gamma Production by the Human Preimplantation Embryo
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