Neurone-specific enolase and Sangtec 100 assays during cardiac surgery: part I - the effects of heparin, protamine and propofol

Neurone-specific enolase (NSE) and Sangtec 100 (S-100) (Sangtec Medical, Sweden) assays are designed for clotted samples, but when studying cerebral damage following cardiac surgery, perioperative samples will contain heparin and/or protamine. The lipid emulsion propofol is also frequently used duri...

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Bibliographic Details
Published in:Perfusion 1997-05, Vol.12 (3), p.163-165
Main Authors: Gao, F, Harris, D NF, Sapsed-Byrne, S, Sharp, S
Format: Article
Language:English
Subjects:
Anesthetics, Intravenous - pharmacology
Anesthetics, Intravenous - therapeutic use
Anticoagulants - pharmacology
Anticoagulants - therapeutic use
Blood Coagulation
Cardiopulmonary Bypass
Cerebrovascular Disorders - blood
Cerebrovascular Disorders - diagnosis
False Negative Reactions
False Positive Reactions
Hemolysis
Heparin - pharmacology
Heparin - therapeutic use
Humans
Intraoperative Care
Intraoperative Complications - blood
Intraoperative Complications - diagnosis
Nerve Tissue Proteins - blood
Organ Specificity
Phosphopyruvate Hydratase - blood
Propofol - pharmacology
Propofol - therapeutic use
Protamines - pharmacology
Protamines - therapeutic use
Radioimmunoassay
Reagent Kits, Diagnostic
S100 Proteins - blood
Sensitivity and Specificity
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Summary:Neurone-specific enolase (NSE) and Sangtec 100 (S-100) (Sangtec Medical, Sweden) assays are designed for clotted samples, but when studying cerebral damage following cardiac surgery, perioperative samples will contain heparin and/or protamine. The lipid emulsion propofol is also frequently used during cardiac surgery and could affect the assays. We, therefore, studied the effects of heparin, protamine and propofol on the accuracy of NSE and S-100 assays in five healthy patients. Blood samples were taken and divided into four groups: normal saline was added to group A; heparin to group B; heparin followed by protamine to group C; and propofol to group D. NSE and S-100 concentrations were measured for all samples. Neither heparin, protamine nor propofol affected the accuracy of S-100 and NSE assays; therefore, samples can be taken throughout operations involving cardiopulmonary bypass without influencing the results.
ISSN:0267-6591
1477-111X
DOI:10.1177/026765919701200303