Characterization of the Osteoclast Ruffled Border Chloride Channel and Its Role in Bone Resorption

Bone resorption by osteoclasts requires massive transcellular acid transport, which is accomplished by the parallel action of a V-type proton pump and a chloride channel in the osteoclast ruffled border. We have studied the molecular basis for the appearance of acid transport as avian bone marrow mo...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1997-07, Vol.272 (30), p.18636-18643
Main Authors: Schlesinger, Paul H., Blair, Harry C., Teitelbaum, Steven L., Edwards, John C.
Format: Article
Language:English
Subjects:
Animals
Bone Resorption
Cell Differentiation
Chickens
Chloride Channels - metabolism
Chloride Channels - physiology
Electrophysiology
Female
In Vitro Techniques
Kidney - metabolism
Lipid Bilayers - metabolism
Microsomes - metabolism
Models, Biological
Monocytes - cytology
Monocytes - metabolism
Osteoclasts - metabolism
Proton Pumps - metabolism
RNA, Messenger - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bone resorption by osteoclasts requires massive transcellular acid transport, which is accomplished by the parallel action of a V-type proton pump and a chloride channel in the osteoclast ruffled border. We have studied the molecular basis for the appearance of acid transport as avian bone marrow mononuclear cells acquire a bone resorptive phenotype in vitro. We demonstrate a critical role for regulated expression of a ruffled border chloride channel as the cells become competent to resorb bone. Molecular characterization of the chloride channel shows that it is related to the renal microsomal chloride channel, p64. In planar bilayers, the ruffled border channel is a stilbene sulfonate-inhibitable, outwardly rectifying chloride channel. A mechanism by which outward rectification of the single channel chloride current could allow efficient regulation of acidification by the channel is discussed.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.272.30.18636