Distance-Dependent Electron Transfer in DNA Hairpins

The distance dependence of photoinduced electron transfer in duplex DNA was determined for a family of synthetic DNA hairpins in which a stilbene dicarboxamide forms a bridge connecting two oligonucleotide arms. Investigation of the fluorescence and transient absorption spectra of these hairpins est...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1997-08, Vol.277 (5326), p.673-676
Main Authors: Lewis, Frederick D., Wu, Taifeng, Zhang, Yifan, Letsinger, Robert L., Greenfield, Scott R., Wasielewski, Michael R.
Format: Article
Language:English
Subjects:
Absorption spectra
Analytical, structural and metabolic biochemistry
Anions
Base Composition
Biological and medical sciences
Chemistry
Coordination chemistry
Deoxyribonucleic acid
DNA
DNA - chemistry
Dna, deoxyribonucleoproteins
Donors
Electron transfer
Electrons
Family (Sociological Unit)
Fluorescence
Fundamental and applied biological sciences. Psychology
Kinetics
Light
Line spectra
Measurement
Models, Molecular
Nucleic Acid Conformation
Nucleic acids
Observation
Organic Chemistry
Oxidation-Reduction
Oxidation-reduction reaction
Oxidation-reduction reactions
Singlet state
Spectrometry, Fluorescence
Spectrum Analysis
Stilbenes
Stilbenes - chemistry
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Summary:The distance dependence of photoinduced electron transfer in duplex DNA was determined for a family of synthetic DNA hairpins in which a stilbene dicarboxamide forms a bridge connecting two oligonucleotide arms. Investigation of the fluorescence and transient absorption spectra of these hairpins established that no photoinduced electron transfer occurs for a hairpin that has six deoxyadenosine-deoxythymidine base pairs. However, the introduction of a single deoxyguanosine-deoxycytidine base pair resulted in distance-dependent fluorescence quenching and the formation of the stilbene anion radical. Kinetic analysis suggests that duplex DNA is somewhat more effective than proteins as a medium for electron transfer but that it does not function as a molecular wire.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.277.5326.673