Effect of blood storage on erythrocyte/wall interactions: implications for surface charge and rigidity

In this report, we study, under flow conditions, the interactions of stored erythrocytes with an artificial surface: a microelectrode whose charge density ranges from -15 to +27 microC/cm2. Interactions consist of red cells slowly circulating on the microelectrode and exerting a real contact with th...

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Bibliographic Details
Published in:European biophysics journal 1997-01, Vol.26 (2), p.175-182
Main Authors: Godin, C, Caprani, A
Format: Article
Language:English
Subjects:
Biophysics
Blood
Blood Preservation
Electric Impedance
Electrodes
Erythrocyte Deformability - physiology
Erythrocyte Membrane - chemistry
Erythrocyte Membrane - physiology
Erythrocytes
Humans
In Vitro Techniques
Membranes
Proteins
Rigidity
Surface Properties
Time Factors
Trypsin - pharmacology
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Summary:In this report, we study, under flow conditions, the interactions of stored erythrocytes with an artificial surface: a microelectrode whose charge density ranges from -15 to +27 microC/cm2. Interactions consist of red cells slowly circulating on the microelectrode and exerting a real contact with the electrode. Interaction is detected and measured by transient fluctuations of the electrolyte resistance obtained by impedance measurement of the microelectrode. Effects of aging induced by storage of whole blood at 4 degrees C show that the surface charge of erythrocytes rapidly decreases when blood is stored for more than 6 days under our experimental conditions. In comparison with trypsin-treated erythrocytes, an eight day storage induces a 60% decrease in the surface charge of red cells. After two weeks of storage, red cells are no longer negatively charged, presumably because of removal of sialic acid. Cells rigidity is significant after 6 days of storage and influences the electrical contact. Membrane rigidity increase could arise from the surface charge decrease. Finally the surface charge decrease could be importance in the use of stored blood.
ISSN:0175-7571
1432-1017
DOI:10.1007/s002490050069