Laminin Peptide Ameliorates Brain Injury by Inhibiting Leukocyte Accumulation in a Rat Model of Transient Focal Cerebral Ischemia

Postischemic cerebral inflammation has been reported to contribute to ischemic brain damage. During inflammation, constituents of the extracellular matrix such as fibronectin and laminin are recognized by certain integrins or proteoglycans and play an important role in the cell adhesion process. The...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cerebral blood flow and metabolism 1997-06, Vol.17 (6), p.605-611
Main Authors: Yanaka, Kiyoyuki, Camarata, Paul J., Spellman, Stephen R., Skubitz, Amy P. N., Furcht, Leo T., Low, Walter C.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biological and medical sciences
Cerebral Cortex - enzymology
Inflammation - therapy
Ischemic Attack, Transient - pathology
Ischemic Attack, Transient - physiopathology
Ischemic Attack, Transient - therapy
Laminin - pharmacology
Leukocyte Count - drug effects
Male
Medical sciences
Neuropharmacology
Neuroprotective agent
Peptide Fragments - genetics
Peptide Fragments - pharmacology
Peroxidase - drug effects
Pharmacology. Drug treatments
Rats
Rats, Sprague-Dawley
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Postischemic cerebral inflammation has been reported to contribute to ischemic brain damage. During inflammation, constituents of the extracellular matrix such as fibronectin and laminin are recognized by certain integrins or proteoglycans and play an important role in the cell adhesion process. The purpose of this study was to evaluate the efficacy of peptides derived from laminin on leukocyte accumulation, infarct size, and neurological outcome in rats subjected to 1 h of cerebral ischemia and 48 h of reperfusion. Forty-four animals were included in this study: transient ischemia without treatment (Group I), treatment with TG-1 peptide (Group II), GD-1 peptide (Group III), and GD-6 peptide (Group IV). Group II showed a significant reduction of the leukocyte accumulation (p < 0.001) and infarct size (p = 0.015) when compared with Group I. The neurological grade of Group II was also significantly better than in Group I at 48 h after reperfusion (p = 0.012). Based on these data, which are the first to explore the therapeutic potential of this peptide in cerebral ischemia, laminin peptide may offer a novel therapeutic approach to allaying injury in ischemic stroke.
ISSN:0271-678X
1559-7016
DOI:10.1097/00004647-199706000-00002