Effects of baicalein isolated from Scutellaria baicalensis on interleukin 1β- and tumor necrosis factor α-induced adhesion molecule expression in cultured human umbilical vein endothelial cells

• Published in: Journal of ethnopharmacology 1997-06, Vol.57 (1), p.63-67
• Main Authors: Kimura, Yoshiyuki, Matsushita, Nobutoshi, Okuda, Hiromichi
• Format: Article
• Language: English
• Subjects: Baicalein; Biological and medical sciences; Cell Adhesion Molecules - biosynthesis; Cell Survival - drug effects; Cells, Cultured; Cytokine; E-Selectin - biosynthesis; Endothelial leukocyte adhesion molecule-1; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Enzyme Inhibitors - isolation & purification; Enzyme Inhibitors - pharmacology; Female; Flavanones; Flavonoids - isolation & purification; Flavonoids - pharmacology; General pharmacology; Human umbilical vein endothelial cells; Humans; Intercellular adhesion molecule-1; Intercellular Adhesion Molecule-1 - biosynthesis; Interleukin-1 - antagonists & inhibitors; Interleukin-1 - pharmacology; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plants, Medicinal - chemistry; Pregnancy; Protein Kinase C - antagonists & inhibitors; Scutellaria baicalensis; Topoisomerase I Inhibitors; Tumor Necrosis Factor-alpha - antagonists & inhibitors; Tumor Necrosis Factor-alpha - pharmacology; Umbilical Veins - cytology; Umbilical Veins - metabolism
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/S0378-8741(97)00045-7

We examined the effects of nine flavonoids isolated from Scutellariae radix on interleukin-1β (IL-1β)- and tumor necrosis factor-α (TNF-α)- induced adhesion molecule expression in cultured human umbilical vein endothelial cells (HUVECs). Among them, we found that baicalein (5,6,7-trihydroxy flavone) dose-dependently inhibited IL-1β and TNF-α-induced endothelial leukocyte adhesion molecule-1 (ELAM-1) and intercellular adhesion molecule-1 (ICAM-1) expressions. Its 50% inhibitory concentrations (IC50) for the IL-1β-induced ELAM-1 and ICAM-1 expressions were 2.3×10−5 M and 4.0×10−5 M, respectively. The IC50 for the TNF-α-induced ELAM-1 and ICAM-1 expressions were 1.5×10−5 M and 3.1×10−5 M, respectively. In addition, protein C-kinase (PKC) inhibitor H7 also inhibited the ELAM-1 and ICAM-1 expressions induced by IL-1β and TNF-α.