The effect of interferon-γ on genetic immunization

• Published in: Vaccine 1997-06, Vol.15 (8), p.896-898
• Main Authors: Xiang, Zhi Quan, He, Zhenning, Wang, Yijie, Ertl, Hildegund C.J.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Viral - biosynthesis; Antigens, Viral - genetics; Antigens, Viral - immunology; Antiviral Agents - pharmacology; Applied microbiology; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; Biological and medical sciences; cytokines; DNA vaccine; DNA, Viral - immunology; Fundamental and applied biological sciences. Psychology; Glycoproteins - genetics; Glycoproteins - immunology; Interferon-gamma - pharmacology; Kinetics; Mice; Microbiology; Promoter Regions, Genetic; rabies virus; Rabies virus - genetics; Rabies virus - immunology; T-Lymphocytes, Helper-Inducer - drug effects; T-Lymphocytes, Helper-Inducer - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, DNA - immunology; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Virology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(96)00269-1

The effect of co-inoculation of a plasmid vector expressing the rabies virus glycoprotein and an additional vector encoding mouse interferon (IFN)-γ on the development of an antigen specific B and T helper cell response was tested upon intramuscular inoculation of mice. The effect of IFN-γ was dependent on the promoter driving expression of the viral antigen. The immune responses to antigen-expressing vector carrying a viral promoter such as the SV40 early promoter or the major histocompatibility (MHC) class I promoter were reduced in presence of IFN-γ while the B and T helper cell response to a vector expressing the antigen under the control of the MHC class II promoter was not affected by this cytokine.