Bidirectional Transport by Distinct Populations of COPI-Coated Vesicles

Electron microscope immunocytochemistry reveals that both anterograde-directed (proinsulin and VSV G protein) and retrograde-directed (the KDEL receptor) cargo are present in COPI-coated vesicles budding from every level of the Golgi stack in whole cells; however, they comprise two distinct populati...

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Bibliographic Details
Published in:Cell 1997-07, Vol.90 (2), p.335-349
Main Authors: Orci, Lelio, Stamnes, Mark, Ravazzola, Mariella, Amherdt, Mylène, Perrelet, Alain, Söllner, Thomas H, Rothman, James E
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Biological Transport - physiology
Cell-Free System
Cells, Cultured
Coated Vesicles - chemistry
Coated Vesicles - metabolism
Coated Vesicles - ultrastructure
Coatomer Protein
Golgi Apparatus - chemistry
Golgi Apparatus - metabolism
Golgi Apparatus - ultrastructure
Membrane Proteins - analysis
Membrane Proteins - metabolism
Microscopy, Immunoelectron
Pancreas - cytology
Rats
Receptors, Peptide - analysis
vesicular stomatitis virus
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Summary:Electron microscope immunocytochemistry reveals that both anterograde-directed (proinsulin and VSV G protein) and retrograde-directed (the KDEL receptor) cargo are present in COPI-coated vesicles budding from every level of the Golgi stack in whole cells; however, they comprise two distinct populations that together can account for at least 80% of the vesicles budding from Golgi cisternae. Segregation of anterograde- from retrograde-directed cargo into distinct sets of COPI-coated vesicles is faithfully reproduced in the cell-free Golgi transport system, in which VSV G protein and KDEL receptor are packaged into separable vesicles, even when budding is driven by highly purified coatomer and a recombinant ARF protein.
ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(00)80341-4