Genes for Tfb2, Tfb3, and Tfb4 subunits of yeast transcription/repair factor IIH: homology to human cyclin-dependent kinase activating kinase and IIH subunits

• Published in: The Journal of biological chemistry 1997-08, Vol.272 (31), p.19319-19327
• Main Authors: Feaver, W.J. (Stanford University School of Medicine, Stanford, CA.), Henry, N.L, Wang, Z, Wu, X, Svejstrup, J.Q, Bushnell, D.A, Friedberg, E.C, Kornberg, R.D
• Format: Article
• Language: English
• Subjects: ADN; Amino Acid Sequence; AMINO ACID SEQUENCES; ANTIBODIES; ANTICORPS; ANTICUERPOS; Base Sequence; Cell Survival; CHEMICAL COMPOSITION; COMPARISONS; COMPLEMENTATION; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; Cyclin-Dependent Kinases - metabolism; DELETIONS; DNA; DNA REPAIR; FACTEUR DE TRANSCRIPTION; FACTORES DE TRANSCRIPCION; GENBANK/U62804; GENBANK/U62805; GENE; GENERO HUMANO; GENES; GENETICA; GENETICS; GENETIQUE; GENRE HUMAIN; Humans; INHIBICION; INHIBITION; MANKIND; MOLECULAR SEQUENCE DATA; MUTANT; MUTANTES; MUTANTS; NUCLEOTIDE EXCISION REPAIR; NUCLEOTIDE SEQUENCE; OPEN READING FRAMES; PROTEIN KINASE; PROTEINA QUINASA; PROTEINE KINASE; SACCHAROMYCES CEREVISIAE; SECUENCIA NUCLEOTIDICA; Sequence Homology, Amino Acid; SEQUENCE NUCLEOTIDIQUE; STRUCTURAL GENES; TFB2 GENE; TFB3 GENE; TRANSCRIPCION; TRANSCRIPTION; Transcription Factor TFIIH; TRANSCRIPTION FACTORS; Transcription Factors - chemistry; Transcription Factors - genetics; Transcription Factors, TFII; VIABILIDAD; VIABILITE; VIABILITY
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.272.31.19319

Genes for the Tfb2, Tfb3, and Tfb4 subunits of yeast RNA polymerase transcription factor IIH (TFIIH) are described. All three genes are essential for cell viability, and antibodies against Tfb3 specifically inhibit transcription in vitro. A C-terminal deletion of Tfb2 caused a defect in nucleotide excision repair, as shown by UV sensitivity of the mutant strain and loss of nucleotide excision repair activity in cell extracts (restored by the addition of purified TFIIH). An interaction between Tfb3 and the Kin28 subunit of TFIIH was detected by the two-hybrid approach, consistent with a role for Tfb3 in linking kinase and core domains of the factor. The deduced amino acid sequence of Tfb2 is similar to that of the 52-kDa subunit of human TFIIH, while Tfb3 is identified as a RING finger protein homologous to the 36-kDa subunit of murine CAK (cyclin-dependent kinase activating kinase) and to the 32-kDa subunit of human TFIIH. Tfb4 is homologous to p34 of human TFIIH and is identified as the weakly associated 37-kDa subunit of the yeast factor. These and other findings reveal a one-to-one correspondence and high degree of sequence similarity between the entire set of yeast and human TFIIH polypeptides.