Inhibition of smooth muscle cell migration by the p21 cyclin-dependent kinase inhibitor (Cip1)

In vascular smooth muscle cells (SMCs), proliferation and migration contribute to lesion formation after arterial injury. In the cell cycle, several cyclin-dependent kinases (cdks) inhibitors are implicated in the regulating of cyclin–cdk activity such as p21Cip1, p16Ink4 and p27Kip1. Although Cip1...

Full description

Saved in:
Bibliographic Details
Published in:Atherosclerosis 1997-07, Vol.132 (1), p.53-59
Main Authors: Fukui, Ryousuke, Shibata, Nobuhiko, Kohbayashi, Eiko, Amakawa, Masahiro, Furutama, Daisuke, Hoshiga, Masaaki, Negoro, Nobuyuki, Nakakouji, Takahiro, Ii, Masaaki, Ishihara, Tadashi, Ohsawa, Nakaaki
Format: Article
Language:English
Subjects:
Actins - analysis
Adhesion
Animals
Aorta
Atherosclerosis (general aspects, experimental research)
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Cell Adhesion
Cell Division
Cell Movement
Cells, Cultured
Cyclin-Dependent Kinase Inhibitor p21
Cyclins - genetics
Cyclins - physiology
Cytoskeleton - chemistry
Medical sciences
Migration
Muscle, Smooth, Vascular - cytology
p21Cip1
Rabbits
Receptors, Fibronectin - analysis
Recombinant Fusion Proteins - physiology
Signal Transduction
Smooth muscle cells
Transfection
Vinculin - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In vascular smooth muscle cells (SMCs), proliferation and migration contribute to lesion formation after arterial injury. In the cell cycle, several cyclin-dependent kinases (cdks) inhibitors are implicated in the regulating of cyclin–cdk activity such as p21Cip1, p16Ink4 and p27Kip1. Although Cip1 inhibits SMC proliferation, its effects on SMC migration are unknown. To test the hypothesis that Cip1 inhibits SMCs migration and proliferation, we transfected the Cip1 gene into a strain of rabbit aortic SMCs (SM3 cells). Both the spreading and the attachment of Cip1-transfected SM3 cells to extracellular matrices (ECMs) were inhibited compared to that of vector-transfected cells. In the modified Boyden's chamber assay the effect of fibronectin on the migratory activity of Cip1-transfected SM3 cells was significantly less than that of vector transfected cells in response to PDGF-BB. These data suggested that Cip1 inhibited both the migration and proliferation of SMC.
ISSN:0021-9150
1879-1484
DOI:10.1016/S0021-9150(97)00086-5