No influence of a single bout of exercise on urinary excretion of 8‐hydroxy‐deoxyguanosine in humans

• Published in: Biochemistry and molecular biology international 1997-07, Vol.42 (3), p.601-609
• Main Authors: Sumida, Satoshi, Okamura, Koji, Doi, Tatsuya, Sakurai, Masao, Yoshioka, Yasuyuki, Sugawa‐Katayama, Yohko
• Format: Article
• Language: English
• Subjects: 8‐hydroxy‐deoxyguanosine; Adolescent; Adult; Creatine Kinase - blood; Creatinine - urine; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - urine; DNA Damage; exercise; Exercise Test; Humans; Male; oxidative DNA damage; Oxidative Stress; Physical Exertion - physiology; Physical Fitness; Running; urine
• ISSN: 1521-6543; 1039-9712; 1521-6551
• DOI: 10.1080/15216549700203011

We investigated the effect of a single bout of intensive exercise on the excretion of 8‐hydroxy‐deoxyguanosine in the 24 h urine from healthy non‐smokers. We studied three exercise tests in Experiment 1; which consisted of incremental exercise to exhaustion on a treadmill in eleven male long distance runners. Experiment 2; which comprised incremental exercise until reaching exhaustion on abicycle ergometer in six male untrained subjects. Experiment 3; which consisted of a 20 km run by eleven male long distance runners. No differences in the urinary 8‐hydroxy‐deoxyguanosine excretion were obselwed from days 1 or 3 after each respective exercise regimen. However, significant increases in the plasma creatine kinase activity were observed at 24 h or 48 h after exercise, except for Experiment 2. Our results thus suggest that the oxidative stress during a single bout of intensive exercise does not result in an accumulation of oxidative DNA damage.