Preincubation of thymocytes with 1‐(5‐isoquinolinesulfonyl)‐2‐methylpiperazine dihydrochloride (H‐7) induces apoptosis in non‐stimulated thymocytes

• Published in: Biochemistry and molecular biology international 1997-07, Vol.42 (3), p.433-441
• Main Authors: Sakurai, Yoshiaki, Hirata, Chihiro, Onishi, Yoshiaki, Tanimoto, Yutaka, Yamada, Takeshi, Kizaki, Harutoshi
• Format: Article
• Language: English
• Subjects: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine - analogs & derivatives; 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine - pharmacology; Animals; apoptosis; Apoptosis - drug effects; Benzoquinones; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Calcium-Calmodulin-Dependent Protein Kinases - antagonists & inhibitors; Cells, Cultured; Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors; Cycloheximide - pharmacology; Dactinomycin - pharmacology; Enzyme Inhibitors - pharmacology; H‐7; Isoenzymes - antagonists & inhibitors; Isoenzymes - physiology; Isoquinolines - pharmacology; Lactams, Macrocyclic; Male; Mice; Mice, Inbred BALB C; Nucleic Acid Synthesis Inhibitors - pharmacology; Phosphorylation - drug effects; protein kinase C; Protein Kinase C - antagonists & inhibitors; Protein Kinase C - physiology; protein kinases; protein phosphorylation; Protein Processing, Post-Translational - drug effects; Protein Synthesis Inhibitors - pharmacology; Quinones - pharmacology; Rifabutin - analogs & derivatives; RNA - biosynthesis; Sulfonamides; T-Lymphocytes - drug effects; thymocytes; Thymus Gland - cytology
• ISSN: 1521-6543; 1039-9712; 1521-6551
• DOI: 10.1080/15216549700202841

1‐(5‐Isoquinolinesulfonyl)‐2‐methylpiperazine hydrochloride (H‐7), an inhibitor of protein kinases, has been shown to inhibit the thymocyte apoptosis induced by various apoptogenic agents. In the present study, when mouse thymocytes were pretreated with H‐7, washed, and cultured for an additional time, apoptosis was induced depending on the preincubation time and the dose of H‐7. The protein kinase C activity in the H‐7‐pretreated and ‐washed cells was not altered, suggesting that an alteration of a certain PKC isoform is related to both the triggering and the progression of apoptosis.