Sequence of centromere separation: characterization of multicentric chromosomes in a rat cell line

The B1 cell line of rat cerebral endothelium origin exhibits several dicentric and multicentric chromosomes. These chromosomes, unlike multicentrics in mouse (Vig and Zinkowski 1986) do not show premature centromere separation. All centromeres deposit kinetochore proteins and appear to be functional...

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Bibliographic Details
Published in:Chromosoma 1989-06, Vol.98 (1), p.13-22
Main Authors: BROCCOLI, C, PAWELETS, N, VIG, B. K
Format: Article
Language:English
Subjects:
Animals
Azure Stains
Biological and medical sciences
Bromodeoxyuridine
Cell Line
Centromere - ultrastructure
Chromosomes - ultrastructure
Cytogenetics
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Karyotyping
Microscopy, Electron
Rats
Vertebrata
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Summary:The B1 cell line of rat cerebral endothelium origin exhibits several dicentric and multicentric chromosomes. These chromosomes, unlike multicentrics in mouse (Vig and Zinkowski 1986) do not show premature centromere separation. All centromeres deposit kinetochore proteins and appear to be functional. Even the centromeres which fail to migrate to the poles during anaphase and make side arm bridges bind to spindle microtubules. Some multicentric chromosomes show kinetochores spaced apart with intervening stretches of euchromatin while others are located adjacent to each other thus exhibiting tandem repeats and forming a "compound" kinetochore (Brinkeley et al. 1984). Also, unlike mouse multicentric chromosomes in which different pericentric regions and the centromeres replicate at different times, the rat chromosomes appear to replicate all pericentric and centric regions in a given multicentric simultaneously. The present studies indicate that centromeres in rat and mouse replicate during the last part of the S-phase and in continuation with the pericentric heterochromatin.
ISSN:0009-5915
1432-0886
DOI:10.1007/bf00293330