Estrogen receptor mRNA in mineralized tissues of rainbow trout: calcium mobilization by estrogen

RT-PCR was undertaken on total RNA extracts from bone and scales of the rainbow trout, Oncorhynchus mykiss. The rainbow trout estrogen receptor (ER)-specific primers used amplified a single product of expected size from each tissue which, using Southern blotting, strongly hybridized with a 32P-label...

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Bibliographic Details
Published in:FEBS letters 1997-07, Vol.411 (1), p.145-148
Main Authors: Armour, Kenneth J., Lehane, David B., Pakdel, Farzad, Valotaire, Yves, Russell, R.Graham G., Henderson, Ian W.
Format: Article
Language:English
Subjects:
17β-Estradiol
Animals
Blotting, Northern
Bone
Bone and Bones - drug effects
Bone and Bones - metabolism
Calcium - metabolism
Estradiol - pharmacology
Estrogen receptor
Freshwater
Marine
Oncorhynchus mykiss
Oncorhynchus mykiss - metabolism
Phosphates - metabolism
Polymerase Chain Reaction
Receptors, Estrogen - biosynthesis
Receptors, Estrogen - genetics
RNA, Messenger
Scale
Teleost
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Summary:RT-PCR was undertaken on total RNA extracts from bone and scales of the rainbow trout, Oncorhynchus mykiss. The rainbow trout estrogen receptor (ER)-specific primers used amplified a single product of expected size from each tissue which, using Southern blotting, strongly hybridized with a 32P-labelled rtER probe under stringent conditions. These data provide the first in vivo evidence of ER mRNA in bone and scale tissues of rainbow trout and suggest that the effects of estrogen observed in this study (increased bone mineral and decreased scale mineral contents, respectively) may be mediated directly through ER.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(97)00680-7