Adenovirus-mediated gene transfer of basic fibroblast growth factor induces in vitro angiogenesis

To investigate the possibility of adenovirus-mediated gene transfer in the treatment of vascular occlusive diseases, we constructed a replication-deficient recombinant adenovirus vector coding for human basic fibroblast growth factor (bFGF) and examined its effect on the proliferation and differenti...

Full description

Saved in:
Bibliographic Details
Published in:Atherosclerosis 1997-07, Vol.132 (2), p.199-205
Main Authors: Takahashi, Jun C, Saiki, Masaaki, Miyatake, Shin-Ichi, Tani, Shoichi, Kubo, Hiroaki, Goto, Kazuo, Aoki, Tomokazu, Takahashi, Jun A, Nagata, Izumi, Kikuchi, Haruhiko
Format: Article
Language:English
Subjects:
Adenoviridae
Adenovirus
Angiogenesis
Basic fibroblast growth factor
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Cells, Cultured
Endothelial cells
Endothelium, Vascular - physiology
Fibroblast Growth Factor 2 - physiology
Gene transfer
Gene Transfer Techniques
Genetic Vectors
Humans
Medical sciences
Miscellaneous
Neovascularization, Physiologic - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To investigate the possibility of adenovirus-mediated gene transfer in the treatment of vascular occlusive diseases, we constructed a replication-deficient recombinant adenovirus vector coding for human basic fibroblast growth factor (bFGF) and examined its effect on the proliferation and differentiation of vascular endothelial cells in vitro. Human umbilical vein endothelial cells (HUVECs) were successfully infected with high efficiency and expressed 18 kD protein which is immunoreactive to anti-bFGF monoclonal antibody. This protein was accumulated mainly in the nuclei of the cells, but was also detected in the culture medium although the complimentary DNA (cDNA) did not contain the classical secreting signal sequence. The proliferation assay of HUVECs infected with bFGF-expressing adenovirus revealed a significant increase in cell number over control. Infection with this virus also enhanced tubular formation of HUVECs on reconstituted basement membrane. Neovascularization and the formation of collateral vessels play important roles in minimizing tissue damage in ischemic disorders. These results imply that the use of bFGF-expressing recombinant adenovirus may be a suitable in vivo gene therapy for ischemic diseases.
ISSN:0021-9150
1879-1484
DOI:10.1016/S0021-9150(97)00102-0