Adenoviral Vector-Mediated Expression of B-50/GAP-43 Induces Alterations in the Membrane Organization of Olfactory Axon Terminals In Vivo

B-50/GAP-43 is an intraneuronal membrane-associated growth cone protein with an important role in axonal growth and regeneration. By using adenoviral vector-directed expression of B-50/GAP-43 we studied the morphogenic action of B-50/GAP-43 in mature primary olfactory neurons that have established f...

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Bibliographic Details
Published in:The Journal of neuroscience 1997-09, Vol.17 (17), p.6575-6586
Main Authors: Holtmaat, Anthony J. G. D, Hermens, Wim T. J. M. C, Sonnemans, Marc A. F, Giger, Roman J, Van Leeuwen, Fred W, Kaplitt, Michael G, Oestreicher, A. Beate, Gispen, Willem Hendrik, Verhaagen, Joost
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Animals
Axons - physiology
Chlorocebus aethiops
Epithelium - physiology
GAP-43 Protein
Genetic Vectors
Membrane Glycoproteins - metabolism
Nerve Endings - physiology
Nerve Tissue Proteins - metabolism
Neuronal Plasticity
Olfactory Pathways - physiology
Synapses - ultrastructure
Synaptic Transmission
Vero Cells
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Summary:B-50/GAP-43 is an intraneuronal membrane-associated growth cone protein with an important role in axonal growth and regeneration. By using adenoviral vector-directed expression of B-50/GAP-43 we studied the morphogenic action of B-50/GAP-43 in mature primary olfactory neurons that have established functional synaptic connections. B-50/GAP-43 induced gradual alterations in the morphology of olfactory synapses. In the first days after overexpression, small protrusions originating from the preterminal axon shaft and from the actual synaptic bouton were formed. With time the progressive formation of multiple ultraterminal branches resulted in axonal labyrinths composed of tightly packed sheaths of neuronal membrane. Thus, B-50/GAP-43 is a protein that can promote neuronal membrane expansion at synaptic boutons. This function of B-50/GAP-43 suggests that this protein may subserve an important role in ongoing structural synaptic plasticity in adult neurons and in neuronal membrane repair after injury to synaptic fields.
ISSN:0270-6474
1529-2401
DOI:10.1523/jneurosci.17-17-06575.1997