Conversion by Peyer's Patch Lymphocytes of Human Enterocytes into M Cells that Transport Bacteria

The epithelium that lines the gut is impermeable to macromolecules and microorganisms, except in Peyer's patches (PPs), where the lymphoid follicle-associated epithelium (FAE) contains M cells that transport antigens and microorganisms. A cultured system that reproduces the main characteristics...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1997-08, Vol.277 (5328), p.949-952
Main Authors: Kernéis, Sophie, Bogdanova, Anna, Kraehenbuhl, Jean-Pierre, Pringault, Eric
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
B lymphocytes
B-Lymphocytes - immunology
Bacteria
Bacterial Translocation
Biochemistry
Biological and medical sciences
Caco 2 cells
Carrier Proteins - analysis
Cell lines
Cell Polarity
Coculture Techniques
Cytology
Digestive system
Enterocytes
Epithelial cells
Fluorescein-5-isothiocyanate
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunity (Disease)
Immunity, Mucosal
Immunobiology
Individualized Instruction
Intestinal Mucosa - cytology
Intestinal Mucosa - immunology
Intestinal Mucosa - microbiology
Intestine. Mesentery
Logical Thinking
Lymphocytes
Lymphocytes - immunology
Mice
Mice, Inbred BALB C
Microfilament Proteins - analysis
Microspheres
Microvilli
Microvilli - chemistry
Microvilli - ultrastructure
Organs and cells involved in the immune response
Peyer's Patches - cytology
Peyer's Patches - immunology
Sucrase-Isomaltase Complex - analysis
T lymphocytes
T-Lymphocytes - immunology
Temperature
Vertebrates: digestive system
Vibrio cholerae
Vibrio cholerae - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The epithelium that lines the gut is impermeable to macromolecules and microorganisms, except in Peyer's patches (PPs), where the lymphoid follicle-associated epithelium (FAE) contains M cells that transport antigens and microorganisms. A cultured system that reproduces the main characteristics of FAE and M cells was established by cultivation of PP lymphocytes with the differentiated human intestinal cell line Caco-2. Lymphocytes settled into the epithelial monolayer, inducing reorganization of the brush border and a temperature-dependent transport of particles and Vibrio cholerae. This model system could prove useful for intestinal physiology, vaccine research, and drug delivery studies.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.277.5328.949