Processing and analyzing the mouse temporal bone to identify gross, cellular and subcellular pathology

A technique has been developed for preparing the mouse temporal bone for histopathological examination: first, as a whole mount to detect any gross malformations of the bony or membranous labyrinths; second, in dissected segments to localize damage in the different sensory organs and to quantify sen...

Full description

Saved in:
Bibliographic Details
Published in:Hearing research 1997-07, Vol.109 (1-2), p.34-45
Main Authors: Bohne, Barbara A, Harding, Gary W
Format: Article
Language:English
Subjects:
Animals
Cochlea - pathology
Cochlea - ultrastructure
Hair Cells, Auditory, Inner - pathology
Hair Cells, Auditory, Inner - ultrastructure
Hair Cells, Auditory, Outer - pathology
Hair Cells, Auditory, Outer - ultrastructure
Histological processing
Inner-ear mutation
Mice
Microscopy, Electron
Microscopy, Phase-Contrast
Mouse
Mutation - genetics
Nerve Fibers - ultrastructure
Plastic Embedding
Saccule and Utricle - pathology
Spiral Ganglion - cytology
Spiral Ganglion - pathology
Temporal bone
Temporal Bone - pathology
Temporal Bone - ultrastructure
Tissue Fixation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A technique has been developed for preparing the mouse temporal bone for histopathological examination: first, as a whole mount to detect any gross malformations of the bony or membranous labyrinths; second, in dissected segments to localize damage in the different sensory organs and to quantify sensory- and supporting-cell losses; and finally, in semi-thick and thin sections to identify and characterize subcellular pathology. Examples are given of the successful application of this technique to mice with very different inner-ear problems, including those with an abnormally short cochlear spiral, a defective lateral semicircular canal, abnormal otoliths over the saccular macula, an increased susceptibility to noise damage and those which lack fibroblast growth factor receptor 3.
ISSN:0378-5955
1878-5891
DOI:10.1016/S0378-5955(97)00019-1